NDLI: Molecular photodynamics involved in multi-photon excitation fluorescence microscopy

Content Provider	Springer Nature Link
Author	Mertz, J.
Copyright Year	1998
Abstract	We present a simple model for calculating the fluorescence generated by the multi-photon excitation (MPE) of molecules in solution. The model takes into account internal molecular dynamics such as ground-state depletion due to inter-system crossing (ISC), as well as external molecular dynamics associated with diffusion into and out of an excitation volume confined in 3-dimensions. Internal and external molecular dynamics are combined by using a technique of linearization of a modified diffusion equation which takes into account the possibility of concentration depletion due to photobleaching. In addition, we discuss the phenomenon of pulse saturation which effectively limits the molecular excitation rate constant in the case of short pulsed excitation. Our results are specifically applied in the context of fluorescence autocorrelation functions and single-molecule detection. In the latter case, we discuss some consequences of high-order multi-photon photobleaching. Finally, we include three appendices to rigorously define the temporal and spatial profiles of an arbitrary excitation beam, and also to discuss some properties of an exact evaluation of concentration depletion due to photobleaching.
Starting Page	53
Ending Page	66
Page Count	14
File Format	PDF
ISSN	14346060
Journal	The European Physical Journal D - Atomic, Molecular, Optical and Plasma Physics
Volume Number	3
Issue Number	1
e-ISSN	14346079
Language	English
Publisher	EDP Sciences, Springer-Verlag
Publisher Date	1998-08-01
Publisher Place	Les Ulis
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics

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