NDLI: A quantitative transcriptome reference map of the normal human brain

Please wait, while we are loading the content...

A quantitative transcriptome reference map of the normal human brain

Content Provider	Springer Nature Link
Author	Caracausi, Maria Vitale, Lorenza Pelleri, Maria Chiara Piovesan, Allison Bru, Samantha Strippoli, Pierluigi
Copyright Year	2014
Abstract	We performed an innovative systematic meta-analysis of 60 gene expression profiles of whole normal human brain, to provide a quantitative transcriptome reference map of it, i.e. a reference typical value of expression for each of the 39,250 known, mapped and 26,026 uncharacterized (unmapped) transcripts. To this aim, we used the software named Transcriptome Mapper (TRAM), which is able to generate transcriptome maps based on gene expression data from multiple sources. We also analyzed differential expression by comparing the brain transcriptome with those derived from human foetal brain gene expression, from a pool of human tissues (except the brain) and from the two normal human brain regions cerebellum and cerebral cortex, which are two of the main regions severely affected when cognitive impairment occurs, as happens in the case of trisomy 21. Data were downloaded from microarray databases, processed and analyzed using TRAM software and validated in vitro by assaying gene expression through several magnitude orders by ‘real-time’ reverse transcription polymerase chain reaction (RT-PCR). The excellent agreement between in silico and experimental data suggested that our transcriptome maps may be a useful quantitative reference benchmark for gene expression studies related to the human brain. Furthermore, our analysis yielded biological insights about those genes which have an intrinsic over-/under-expression in the brain, in addition offering a basis for the regional analysis of gene expression. This could be useful for the study of chromosomal alterations associated to cognitive impairment, such as trisomy 21, the most common genetic cause of intellectual disability.
Starting Page	267
Ending Page	287
Page Count	21
File Format	PDF
ISSN	13646745
Journal	Neurogenetics
Volume Number	15
Issue Number	4
e-ISSN	13646753
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-09-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human brain Gene expression profile Integrated transcriptome reference map Meta-analysis Neurosciences Human Genetics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Cellular and Molecular Neuroscience Genetics (clinical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Pathway Analysis of the Human Brain Transcriptome in Disease

A molecular view of the normal human thyroid structure and function reconstructed from its reference transcriptome map

Integrated Quantitative Transcriptome Maps of Human Trisomy 21 Tissues and Cells

A novel gene derived from a segmental duplication shows perturbed expression in Alzheimer’s disease

Confirmation of region-specific patterns of gene expression in the human brain

Analysis of gene expression in the nervous system identifies key genes and novel candidates for health and disease

Transcriptional regulation of long-term potentiation

Up-regulation of SNCA gene expression: implications to synucleinopathies

Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis

A quantitative transcriptome reference map of the normal human brain

Similar Documents

Pathway Analysis of the Human Brain Transcriptome in Disease

A molecular view of the normal human thyroid structure and function reconstructed from its reference transcriptome map

Integrated Quantitative Transcriptome Maps of Human Trisomy 21 Tissues and Cells

A novel gene derived from a segmental duplication shows perturbed expression in Alzheimer’s disease

Confirmation of region-specific patterns of gene expression in the human brain

Analysis of gene expression in the nervous system identifies key genes and novel candidates for health and disease

Transcriptional regulation of long-term potentiation

Up-regulation of SNCA gene expression: implications to synucleinopathies

Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis

A quantitative transcriptome reference map of the normal human brain