NDLI: Resolution enhancement of digital laser scanning fluorescence microscopy with a dual-lens optical pickup head

Content Provider	Springer Nature Link
Author	Tsai, Rung Ywan Chen, Jung Po Lee, Yuan Chin Chiang, Hung Chih Huang, Tai Ting Huang, Chun Chieh Cheng, Chih Ming Cheng, Chung Ta Lo, Feng Hsiang Tiao, Golden
Copyright Year	2016
Abstract	The resolution of the cell fluorescence image captured by a digital laser scanning microscopy with a modified dual-lens BD-ROM optical pickup head is enhanced by image registration and double sample frequency. A dual objective lens of red (655 nm) and blue (405 or 488 nm) laser sources with numerical apertures of 0.6 and 0.85 is used for sample focusing and position tracking and cell fluorescence image capturing, respectively. The image registration and capturing frequency are based on the address-coded patterns of a sample slide. The address-coded patterns are designed as a string of binary code, which comprises a plurality of base-straight lands and grooves and data-straight grooves. The widths of the base-straight lands, base-straight grooves, and data-straight grooves are 0.38, 0.38, and 0.76 μm, respectively. The numbers of sample signals in the x-direction are measured at every intersection point by intersecting the base intensity of the push–pull signal of the address-coded patterns, which has a minimum spacing of 0.38 μm. After taking a double sample frequency, the resolution of the measured cell fluorescence image is enhanced from 0.38 μm to the diffraction limit of the objective lens.
Starting Page	817
Ending Page	823
Page Count	7
File Format	PDF
ISSN	13406000
Journal	Optical Review
Volume Number	23
Issue Number	5
e-ISSN	13499432
Language	English
Publisher	Springer Japan
Publisher Date	2016-07-21
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fluorescence image Resolution Digital laser scanning microscopy Address-coded pattern Optical pickup head Optics, Lasers, Photonics, Optical Devices Atomic, Molecular, Optical and Plasma Physics Quantum Optics Microwaves, RF and Optical Engineering
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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