NDLI: Impedimetric biosensor based on bimetallic AgPt nanoparticle-decorated carbon nanotubes as highly conductive film surface

Content Provider	Springer Nature Link
Author	Shiravand, Toran Azadbakht, Azadeh
Copyright Year	2017
Abstract	In the present study, an ultrasensitive impedimetric aptasensor for the quantitative detection of diclofenac (DCF) was fabricated based on a novel signal amplification strategy. As a transducer material, acid-oxidized carbon nanotubes (CNT-COOH) and Pt and Ag nanoparticles (PtAgNPs) were used for promoting electron transfer between the electroactive probe and electrode. It is worth noting that the proposed nanocomposite revealed significant properties through the synergistic effects of the nanocomponent materials. In this strategy, aminated capture probe (ssDNA1) and DCF aptamer (ssDNA2) as a detection probe were attached at the surface of a modified electrode via the formation of covalent amide bond and hybridization, respectively. The proposed nanoaptasensor combined the advantages of the deposition of neatly arranged PtAgNPs and the covalent attachment of the capture probe at the surface of the modified electrode. The change of interfacial charge transfer resistance (R ct ) recorded by [Fe(CN)6]3−/4− as a redox probe was monitored for sensitive quantitative detection of DCF. Addition of DCF resulted in the increase of the value of R ct due to suppression of the electron exchange between [Fe(CN)6]3−/4− in the surface layer. Under optimized conditions, the proposed aptasensor showed good detection range from 10 pM to 800 nM with unprecedented detection limit of 2.8 pM. More importantly, the developed strategy showed highly sensitive responses to DCF, responses that are more sensitive than those of previously reported methods.
Starting Page	1699
Ending Page	1711
Page Count	13
File Format	PDF
ISSN	14328488
Journal	Journal of Solid State Electrochemistry
Volume Number	21
Issue Number	6
e-ISSN	14330768
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-02-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aptamer Platinum-silver nanoparticles Diclofenac Impedimetric Physical Chemistry Electrochemistry Energy Storage Characterization and Evaluation of Materials Analytical Chemistry Condensed Matter Physics
Content Type	Text
Resource Type	Article
Subject	Condensed Matter Physics Materials Science Electrical and Electronic Engineering Electrochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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