NDLI: An amperometric glucose oxidase biosensor based on liposome microreactor-chitosan nanocomposite-modified electrode for determination of trace mercury

Content Provider	Springer Nature Link
Author	Yu, Jia Guan, Huanan Chi, Defu
Copyright Year	2016
Abstract	A biosensor for trace mercury ions based on glucose oxidase (GOD) immobilized on liposome microreactor and chitosan (CS) nanocomposite through layer-by-layer method is described herein. The GOD liposome microreactors (GLMs) were characterized using Fourier transform infrared (FTIR), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and amperometric methods. The results indicated that GLMs were prepared by encapsulating the enzyme GOD in l-α-phosphatidylcholine liposome resulting in spherical bioreactor with a mean diameter of 5.83 ± 0.75 μm. The encapsulation efficiency and drug loading content of the GLMs were about 53.58 ± 0.91 and 41.15 ± 0.95%, respectively. Cyclic voltammogram (CV) was further utilized to explore relevant electrochemical activity on (CS/GLM)8 nanocomposite-modified glassy carbon electrode (GCE). The biosensor based on the (CS/GLM)8-GCE composite films was applied to detect Hg2+ with a broad linear range from 0.5 to 5.00 μmol/L, and the detection limit was brought down to 0.076 μmol/L. The apparent Michaelis-Menten constant, K m, for the enzymatic reaction was 0.37 mmol/L (S/N = 3). Furthermore, the biosensor showed good stability and reproducibility. Such new biosensor based on encapsulation of GOD within liposome microreactors shows great promise for rapid, simple, and cost-effective analysis of Hg2+ in environmental samples.
Starting Page	1175
Ending Page	1183
Page Count	9
File Format	PDF
ISSN	14328488
Journal	Journal of Solid State Electrochemistry
Volume Number	21
Issue Number	4
e-ISSN	14330768
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-11-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosensor Glucose oxidase (GOD) Liposome Layer by layer (LbL) Mercury(II) (Hg2+) Physical Chemistry Electrochemistry Energy Storage Characterization and Evaluation of Materials Analytical Chemistry Condensed Matter Physics
Content Type	Text
Resource Type	Article
Subject	Condensed Matter Physics Materials Science Electrical and Electronic Engineering Electrochemistry

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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