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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Yu Zhu, Guo Fei Ren, Si Yan Han, Yong Guang Luo, Yue Du, Lin Fang |
| Copyright Year | 2013 |
| Abstract | The efficiency and high specificity of tobacco etch virus protease (TEVp) has made it widely used for cleavage of recombinant fusion proteins. However, TEVp suffers from a few intrinsic defects such as self-cleavage, poorly expressed in E. coli and less soluble. So some mutants were designed to improve it, such as S219V, T17S/N68D/I77V and L56V/S135G etc. MD simulations for the WT TEVp and its mutants were performed to explore the underlying dynamic effects of mutations on TEVp. Although the globular domains are fairly conserved, the three mutations have diverse effects on the dynamics properties of TEVp, including the elongation of β-sheet, conversion of loop to helix and the flexibility of active core. Our present study indicates that the three mutants for TEVp can change their secondary structure and tend to form more helixes and sheets to improve stability. The study also helps us to understand the effects of some mutations on TEVp, provides us insights into the change of them at the atomic level and gives a potential rational method to design an improved protein. |
| Starting Page | 4865 |
| Ending Page | 4875 |
| Page Count | 11 |
| File Format | |
| ISSN | 16102940 |
| Journal | Journal of Molecular Modeling |
| Volume Number | 19 |
| Issue Number | 11 |
| e-ISSN | 09485023 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2013-09-17 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Molecular dynamics simulations Mutation Stability Tobacco etch virus protease Computer Applications in Chemistry Molecular Medicine Computer Application in Life Sciences Characterization and Evaluation of Materials Theoretical and Computational Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Physical and Theoretical Chemistry Computational Theory and Mathematics Catalysis Inorganic Chemistry Computer Science Applications |
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