NDLI: Natronobacillus azotifigens gen. nov., sp. nov., an anaerobic diazotrophic haloalkaliphile from soda-rich habitats

Content Provider	Springer Nature Link
Author	Sorokin, I. D. Zadorina, E. V. Kravchenko, I. K. Boulygina, E. S. Tourova, T. P. Sorokin, D. Y.
Copyright Year	2008
Abstract	Gram-positive bacteria capable of nitrogen fixation were obtained in microoxic enrichments from soda soils in south-western Siberia, north-eastern Mongolia, and the Lybian desert (Egypt). The same organisms were obtained in anoxic enrichments with glucose from soda lake sediments in the Kulunda Steppe (Altai, Russia) using nitrogen-free alkaline medium of pH 10. The isolates were represented by thin motile rods forming terminal round endospores. They are strictly fermentative saccharolytic anaerobes but tolerate high oxygen concentrations, probably due to a high catalase activity. All of the strains are obligately alkaliphilic and highly salt-tolerant natronophiles (chloride-independent sodaphiles). Growth was possible within a pH range from 7.5 to 10.6, with an optimum at 9.5–10, and within a salt range from 0.2 to 4 M Na+, with an optimum at 0.5–1.5 M for the different strains. The nitrogenase activity in the whole cells also had an alkaline pH optimum but was much more sensitive to high salt concentrations compared to the growing cells. The isolates formed a compact genetic group with a high level of DNA similarity. Phylogenetic analysis based on 16S-rRNA gene sequences placed the isolates into Bacillus rRNA group 1 as a separate lineage with Amphibacillus tropicus as the nearest relative. In all isolates the key functional nitrogenase gene nifH was detected. A new genus and species, Natronobacillus azotifigens gen. nov., sp. nov., is proposed to accommodate the novel diazotrophic haloalkaliphiles.
Starting Page	819
Ending Page	827
Page Count	9
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	12
Issue Number	6
e-ISSN	14334909
Language	English
Publisher	Springer Japan
Publisher Date	2008-09-04
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nitrogen fixation Soda soils Soda lakes Haloalkaliphilic nifH gene Bacillus rRNA group 1 Ecology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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