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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhi, Xiao Yang Yang, Ling Ling Wu, Jin Yuan Tang, Shu Kun Li, Wen Jun |
| Copyright Year | 2007 |
| Abstract | The genera Actinopolyspora and Streptomonospora are two groups of extremely halophilic filamentous actinomycetes. Members of these two genera are isolated frequently, probably due to the high occurrence of these actinomycetes in the hypersaline soil environment. Although members of these genera can be identified by micromorphological criteria, the extensive chemotaxonomic characterization of each new isolates is a time-consuming task which cannot always be undertaken when handling large numbers of isolates as is the case in natural products screening programmes. In this work, the design of one set of genus-specific PCR primers which allows rapid detection of members of the genus Actinopolyspora by means of PCR amplification is presented. And we developed a multiplex PCR protocol for identification of the species of the genera Actinopolyspora and Streptomonospora, simultaneously. |
| Starting Page | 543 |
| Ending Page | 548 |
| Page Count | 6 |
| File Format | |
| ISSN | 14310651 |
| Journal | Extremophiles |
| Volume Number | 11 |
| Issue Number | 3 |
| e-ISSN | 14334909 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2007-02-17 |
| Publisher Place | Tokyo |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Actinopolyspora Streptomonospora Genus-specific primers Multiplex PCR Identification Ecology Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Medicine Microbiology |
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