NDLI: Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd2+ stress: evidence from transcriptional, enzymatic, and metabolic profiles

Please wait, while we are loading the content...

Structure of dimeric, recombinant Sulfolobus solfataricus phosphoribosyl diphosphate synthase: a bent dimer defining the adenine specificity of the substrate ATP

Characterization of the amino acid residues mediating the unique amino-sugar-1-phosphate acetyltransferase activity of the archaeal ST0452 protein

Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd2+ stress: evidence from transcriptional, enzymatic, and metabolic profiles

Comparison of the microbial communities of hot springs waters and the microbial biofilms in the acidic geothermal area of Copahue (Neuquén, Argentina)

UDP-sulfoquinovose formation by Sulfolobus acidocaldarius

Identification and characterization of 2-keto-3-deoxy-l-rhamnonate dehydrogenase belonging to the MDR superfamily from the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans: implications to l-rhamnose metabolism in archaea

Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1

Bioreduction and immobilization of hexavalent chromium by the extremely acidophilic Fe(III)-reducing bacterium Acidocella aromatica strain PFBC

Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus

Preparation of poly(3-hydroxybutyrate-co-hydroxyvalerate) films from halophilic archaea and their potential use in drug delivery

Culturable diversity of aerobic halophilic archaea (Fam. Halobacteriaceae) from hypersaline, meromictic Transylvanian lakes

A standardized protocol for the UV induction of Sulfolobus spindle-shaped virus 1

Extremophiles : Volume 19, Issue 1, January 2015

Extremophiles : Volume 18

Extremophiles : Volume 17

Extremophiles : Volume 16

Extremophiles : Volume 15

Extremophiles : Volume 14

Extremophiles : Volume 13

Extremophiles : Volume 12

Extremophiles : Volume 11

Extremophiles : Volume 10

Extremophiles : Volume 9

Extremophiles : Volume 8

Extremophiles : Volume 7

Extremophiles : Volume 6

Extremophiles : Volume 5

Extremophiles : Volume 4

Extremophiles : Volume 3

Extremophiles : Volume 2

Extremophiles : Volume 1

Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd2+ stress: evidence from transcriptional, enzymatic, and metabolic profiles

Content Provider	Springer Nature Link
Author	Zheng, Chunli Chen, Minjie Tao, Zhanlong Zhang, Li Zhang, Xue Feng Wang, Jian Ying Liu, Jianshe
Copyright Year	2015
Abstract	Acidithiobacillus ferrooxidans is a heavy metal-tolerant acidophilic chemolithotroph found in acidic mine effluent and is used commercially in the bioleaching of sulfide ores. In this work, we investigated the interplay between divalent cadmium (Cd2+) resistance and expression of genes involved in the sulfur assimilation pathway (SAP). We also investigated the response of the thiol-containing metal-chelating metabolites, cysteine and glutathione(GSH), to increasing Cd2+ concentrations. During growth in the presence of 30 mM Cd2+, the concentrations of mRNA for 5 genes in the SAP pathway increased more than fourfold: these encode ATP sulfurylase (ATPS), adenosine 5′-phosphosulfate (APS) reductase, sulfite reductase (SiR), serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL). Increased transcription was also reflected in increased enzyme activities: those of SAT and adenosylphosphosulfate reductase (APR) reached a peak of 26- and 15.8-fold, respectively, compared to the control culture in the presence of 15 mM Cd2+. In contrast, the activity of OAS-TL, which is responsible for the biosynthesis of cysteine, was diminished. At the metabolite level, the intracellular cysteine and GSH contents nearly doubled. These results suggested that Cd2+ induced transcription of SAP genes, while directly inhibiting the activities of some enzymes (e.g., OAS-TL). Overall, these results are consistent with a detoxification/resistance mechanism involving enhanced sulfur uptake and sequestration of Cd2+ by cysteine and glutathione.
Starting Page	429
Ending Page	436
Page Count	8
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	19
Issue Number	2
e-ISSN	14334909
Language	English
Publisher	Springer Japan
Publisher Date	2015-01-10
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acidithiobacillus ferrooxidans Cadmium Cysteine Gene expression Glutathione Metal resistance Sulfur assimilation Microbiology Biotechnology Biochemistry Microbial Ecology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Interplay Between Expression of Sulfur Assimilation Pathway Genes and Zn2+ and Pb2+ Stress in Acidithiobacillus ferrooxidans

Proteomic Analysis of Differential Protein Expression in Acidithiobacillus ferrooxidans Grown on Ferrous Iron or Elemental Sulfur

Proteomic Analysis of Differential Protein Expression in Acidithiobacillus ferrooxidans Grown on Ferrous Iron or Elemental Sulfur.

Expression, purification and characterization of a cysteine desulfurase, IscS, from Acidithiobacillus ferrooxidans

Differential gene expression in response to copper in Acidithiobacillus ferrooxidans strains possessing dissimilar copper resistance

Gene expression modulation by heat stress in Acidithiobacillus ferrooxidans LR

Effects of Copper Exposure on Expression of Glutathione-Related Genes in Acidithiobacillus ferrooxidans

Expression, purification and characterization of an iron-sulfur cluster assembly protein, IscU, from Acidithiobacillus ferrooxidans

Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis

Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd2+ stress: evidence from transcriptional, enzymatic, and metabolic profiles

Similar Documents

Interplay Between Expression of Sulfur Assimilation Pathway Genes and Zn2+ and Pb2+ Stress in Acidithiobacillus ferrooxidans

Proteomic Analysis of Differential Protein Expression in Acidithiobacillus ferrooxidans Grown on Ferrous Iron or Elemental Sulfur

Proteomic Analysis of Differential Protein Expression in Acidithiobacillus ferrooxidans Grown on Ferrous Iron or Elemental Sulfur.

Expression, purification and characterization of a cysteine desulfurase, IscS, from Acidithiobacillus ferrooxidans

Differential gene expression in response to copper in Acidithiobacillus ferrooxidans strains possessing dissimilar copper resistance

Gene expression modulation by heat stress in Acidithiobacillus ferrooxidans LR

Effects of Copper Exposure on Expression of Glutathione-Related Genes in Acidithiobacillus ferrooxidans

Expression, purification and characterization of an iron-sulfur cluster assembly protein, IscU, from Acidithiobacillus ferrooxidans

Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis

Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd2+ stress: evidence from transcriptional, enzymatic, and metabolic profiles