NDLI: Glutamate restores growth but not motility in the absence of chloride in the moderate halophile Halobacillus halophilus

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Glutamate restores growth but not motility in the absence of chloride in the moderate halophile Halobacillus halophilus

Content Provider	Springer Nature Link
Author	Saum, Stephan H. Roeßler, Markus Koller, Christiane Sydow, Jasmin F. Müller, Volker
Copyright Year	2007
Abstract	Halobacillus halophilus is a strictly chloride-dependent, moderately halophilic bacterium that synthesizes glutamate and glutamine as the major compatible solutes at intermediate NaCl concentrations. The key enzyme in production of the compatible solutes glutamine and glutamate, glutamine synthetase, is dependent on chloride on a transcriptional and activity level. This led us to ask whether exogenous supply of glutamate may relief the chloride dependence of growth of H. halophilus. Growth of H. halophilus in minimal medium at 1 M NaCl was stimulated by exogenous glutamate and transport experiments revealed a chloride-independent glutamate uptake by whole cells. Growth was largely impaired in the absence of chloride and, at the same time, glutamate and glutamine pools were reduced by 90%. Exogenous glutamate fully restored growth, and cellular glutamate and glutamine pools were refilled. Although glutamate could restore growth in the absence of chloride, another chloride-dependent process, flagellum synthesis and motility, was not restored by glutamate. The differential effect of glutamate on the two chloride-dependent processes, growth and motility, indicates that glutamate can not substitute chloride in general but apparently bypasses one function of the chloride regulon, the adjustment of pool sizes of compatible solutes.
Starting Page	711
Ending Page	717
Page Count	7
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	11
Issue Number	5
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-06-13
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chloride Glutamate Halophile Motility Solutes Ecology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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