NDLI: Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus

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Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus

Acknowledgment to reviewers 2008

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Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus

Content Provider	Springer Nature Link
Author	Deng, Ling Zhu, Haojun Chen, Zhengjun Liang, Yun Xiang She, Qunxin
Copyright Year	2009
Abstract	Sulfolobus islandicus is being used as a model for studying archaeal biology, geo-biology and evolution. However, no genetic system is available for this organism. To produce an S. islandicus mutant suitable for genetic analyses, we screened for colonies with a spontaneous pyrEF mutation. One mutant was obtained containing only 233 bp of the original pyrE sequence in the mutant allele and it was used as a host to delete the β-glycosidase (lacS) gene. Two unmarked gene deletion methods were employed, namely plasmid integration and segregation, and marker replacement and looping out, and unmarked lacS mutants were obtained by each method. A new alternative recombination mechanism, i.e., marker circularization and integration, was shown to operate in the latter method, which did not yield the designed deletion mutation. Subsequently, Sulfolobus–E. coli plasmid shuttle vectors were constructed, which genetically complemented ΔpyrEFΔlacS mutation after transformation. Thus, a complete set of genetic tools was established for S. islandicus with pyrEF and lacS as genetic markers.
Starting Page	735
Ending Page	746
Page Count	12
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	13
Issue Number	4
e-ISSN	14334909
Language	English
Publisher	Springer Japan
Publisher Date	2009-06-10
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	pyrEF marker Unmarked gene deletion S. islandicus host–vector system pyrEF lacS deletion mutant Ecology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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Disruption of the gene encoding restriction endonuclease SuaI and development of a host–vector system for the thermoacidophilic archaeon Sulfolobus acidocaldarius

Revealing S-layer Functions in the Hyperthermophilic Crenarchaeon Sulfolobus islandicus

Cell Structure Changes in the Hyperthermophilic Crenarchaeon Sulfolobus islandicus Lacking the S-Layer

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Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus

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Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus

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