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| Content Provider | Springer Nature Link |
|---|---|
| Author | Schmid, A. Kollmer, A. Mathys, R. G. Witholt, B. |
| Copyright Year | 1998 |
| Abstract | Many pseudomonads and other bacteria can grow on aliphatic and aromatic hydrocarbons that occur in the environment. We are examining the potential of such organisms as biocatalysts for the oxidation of a variety of substituted aliphatic and aromatic compounds. To attain a high production rate of oxidation products via such biotransformations, we have focused on two-liquid phase culture systems. In these systems, cells are grown in liquid media consisting of an aqueous phase containing water-soluble growth substrates and droplets of a water-immiscible organic solvent containing bioconversion substrates and products. For industrial applications of such two-liquid phase processes, several questions remain. What are the maximum rates at which apolar compounds can be transferred from the apolar phase to cells growing in the aqueous phase, i.e., what are the maximum space-time yields attainable in two-liquid phase fermentations under practical conditions? What does an efficient downstream processing of two-liquid phase medium involve? What safety regimes should be considered in working with flammable organic solvents? Can elevated pressure be used to increase oxygen transfer? Based on answers to these questions, we have recently developed a high-pressure, explosion-proof bioreactor system with Bioengineering AG (Wald, Switzerland), which will be installed in our pilot plant and used to explore two-liquid phase bioconversions at a pilot scale. |
| Starting Page | 249 |
| Ending Page | 256 |
| Page Count | 8 |
| File Format | |
| ISSN | 14310651 |
| Journal | Extremophiles |
| Volume Number | 2 |
| Issue Number | 3 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 1998-08-20 |
| Publisher Place | Tokyo |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Medicine Microbiology |
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