NDLI: Thermoactive extracellular proteases of Geobacillus caldoproteolyticus, sp. nov., from sewage sludge

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Thermoactive extracellular proteases of Geobacillus caldoproteolyticus, sp. nov., from sewage sludge

Content Provider	Springer Nature Link
Author	Chen, Xiao Ge Stabnikova, Olena Tay, Joo Hwa Wang, Jing Yuan Tay, Stephen Tiong Lee
Copyright Year	2004
Abstract	A proteolytic thermophilic bacterial strain, designated as strain SF03, was isolated from sewage sludge in Singapore. Strain SF03 is a strictly aerobic, Gram stain-positive, catalase-positive, oxidase-positive, and endospore-forming rod. It grows at temperatures ranging from 35 to 65°C, pH ranging from 6.0 to 9.0, and salinities ranging from 0 to 2.5%. Phylogenetic analyses revealed that strain SF03 was most similar to Saccharococcus thermophilus, Geobacillus caldoxylosilyticus, and G. thermoglucosidasius, with 16S rRNA gene sequence identities of 97.6, 97.5 and 97.2%, respectively. Based on taxonomic and 16S rRNA analyses, strain SF03 was named G. caldoproteolyticus sp. nov. Production of extracellular protease from strain SF03 was observed on a basal peptone medium supplemented with different carbon and nitrogen sources. Protease production was repressed by glucose, lactose, and casamino acids but was enhanced by sucrose and NH4Cl. The cell growth and protease production were significantly improved when strain SF03 was cultivated on a 10% skim-milk culture medium, suggesting that the presence of protein induced the synthesis of protease. The protease produced by strain SF03 remained active over a pH range of 6.0–11.0 and a temperature range of 40–90°C, with an optimal pH of 8.0–9.0 and an optimal temperature of 70–80°C, respectively. The protease was stable over the temperature range of 40–70°C and retained 57 and 38% of its activity at 80 and 90°C, respectively, after 1 h.
Starting Page	489
Ending Page	498
Page Count	10
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	8
Issue Number	6
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-08-21
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Characterization Extracellular Geobacillus caldoproteolyticus Thermoactive protease Thermophilic
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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