NDLI: Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125

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Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125

Content Provider	Springer Nature Link
Author	Papa, Rosanna Glagla, Susanne Danchin, Antoine Schweder, Thomas Mari, Gennaro Duilio, Angela
Copyright Year	2006
Abstract	The capability of microorganisms to utilize different carbohydrates as energy source reflects the availability of these substrates in their habitat. Investigation of the proteins involved in carbohydrate usage, in parallel with analysis of their expression, is then likely to provide information on the interaction between microorganisms and their ecosystem. We analysed the growth behaviour of the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 in the presence and in the absence of different carbon source. A marked increase in the optical density was detected when l-malate was added to the growth medium. Bacterial proteins differently expressed in the presence of l-malate were identified by proteomic profiling experiments. On the basis of their relative increase, six proteins were selected for further analyses. Among these, the expression of a putative outer membrane porin was demonstrated to be heavily induced by l-malate. The presence of a functionally active two-component regulatory system very likely controlled by l-malate was found in the upstream region of the porin gene. A non functional genomic porin mutant was then constructed showing a direct involvement of the protein in the uptake of l-malate. To the best of our knowledge, the occurrence of such a regulatory system has never been reported in Pseudoalteromonads so far and might constitute a key step in the development of an effective inducible cold expression system.
Starting Page	483
Ending Page	491
Page Count	9
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	10
Issue Number	6
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-06-22
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Psychrophiles Pseudoalteromonas haloplanktis TAC125 Carbon source response Two-component regulatory system l-malate Ecology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125

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