NDLI: Electron transfer from cytochrome c to cupredoxins

Content Provider	Springer Nature Link
Author	Takayama, Shin ichi J. Irie, Kiyofumi Tai, Hulin Kawahara, Takumi Hirota, Shun Takabe, Teruhiro Alcaraz, Luis A. Donaire, Antonio Yamamoto, Yasuhiko
Copyright Year	2009
Abstract	Electron transfer (ET) through and between proteins is a fundamental biological process. The activation energy for an ET reaction depends upon the Gibbs energy change upon ET (ΔG 0) and the reorganization energy. Here, we characterized ET from Pseudomonas aeruginosa cytochrome c 551 (PA) and its designed mutants to cupredoxins, Silene pratensis plastocyanin (PC) and Acidithiobacillus ferrooxidans rusticyanin (RC), through measurement of pseudo-first-order ET rate constants (k obs). The influence of the ΔG 0 value for ET from PA to PC or RC on the k obs value was examined using a series of designed PA proteins exhibiting a variety of E m values, which afford the ΔG 0 variation range of 58–399 meV. The plots of the k obs values obtained against the ΔG 0 values for both PA–PC and PA–RC redox pairs could be fitted well with a single Marcus equation. We have shown that the ET activity of cytochrome c can be controlled by tuning the E m value of the protein through the substitution of amino acid residues located in hydrophobic-core regions relatively far from the redox center. These findings provide novel insights into the molecular design of cytochrome c, which could be utilized for controlling its ET activity by means of protein engineering.
Starting Page	821
Ending Page	828
Page Count	8
File Format	PDF
ISSN	09498257
Journal	JBIC Journal of Biological Inorganic Chemistry
Volume Number	14
Issue Number	6
e-ISSN	14321327
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-03-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cupredoxin Electron transfer Cytochrome c Marcus theory Redox potential Biochemistry Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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