NDLI: Zinc(II) is required for the in vivo and in vitro folding of mouse copper metallothionein in two domains

Content Provider	Springer Nature Link
Author	Bofill, Roger Capdevila, Mercè Cols, Neus Atrian, Sílvia Gonzàlez Duarte, Pilar
Copyright Year	2001
Abstract	We postulate that zinc(II) is a keystone in the structure of physiological mouse copper metallothionein 1 (Cu-MT 1). Only when Zn(II) is coordinated does the structure of the in vivo- and in vitro-conformed Cu-MT species consist of two additive domains. Therefore, the functionally active forms of the mammalian Cu-MT may rely upon a two-domain structure. The in vitro behaviour of the whole protein is deduced from the Cu titration of the apo and Zn-containing forms and compared with that of the independent fragments using CD, UV-vis, ESI-MS and ICP-AES. We propose the formation of the following Cu,Zn-MT species during Zn/Cu replacement in Zn7-MT: (Zn4)α(Cu4Zn1)β-MT, (Cu3Zn2)α(Cu4Zn1)β-MT and (Cu4Zn1)α(Cu6)β-MT. The cooperative formation of (Cu3Zn2)α(Cu4Zn1)β-MT from (Zn4)α(Cu4Zn1)β-MT indicates that the preference of Cu(I) for binding to the β domain is only partial and not absolute, as otherwise accepted. Homometallic Cu-MT species have been obtained either from the apoform of MT or from Zn7-MT after total replacement of zinc. In these species, copper distribution cannot be inferred from the sum of the independent α and β fragments. The in vivo synthesis of the entire MT in Cu-supplemented media has afforded Cu7Zn3-MT [(Cu3Zn2)α(Cu4Zn1)β-MT], while that of αMT has rendered a mixture of Cu4Zn1-αMT (40%), Cu5Zn1-αMT (20%) and Cu7-αMT (40%). In the case of βMT, a mixture of Cu6-βMT (25%) and Cu7-βMT (75%) was recovered [1]. These species correspond to some of those conformed in vitro and confirm that Zn(II) is essential for the in vivo folding of Cu-MT in a Cu-rich environment. A final significant issue is that common procedures used to obtain mammalian Cu6-βMT from native sources may not be adequate.
Starting Page	405
Ending Page	417
Page Count	13
File Format	PDF
ISSN	09498257
Journal	JBIC Journal of Biological Inorganic Chemistry
Volume Number	6
Issue Number	4
e-ISSN	14321327
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-03-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Copper-metallothionein Zinc-metallothionein Recombinant metallothionein Copper α domain In vivo copper metallothionein Biochemistry Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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