NDLI: Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I

Please wait, while we are loading the content...

A comparison of DNA binding profiles of dinuclear platinum compounds with polyamine linkers and the trinuclear platinum phase II clinical agent BBR3464

Stabilities of lead(II) complexes formed in aqueous solution with methyl thiophosphate (MeOPS2–), uridine 5'-O-thiomonophosphate (UMPS2–) or adenosine 5'-O-thiomonophosphate (AMPS2–)

Chemical shift-based constraints for solution structure determination of paramagnetic low-spin heme proteins with bis-His and His-CN axial ligands: the cases of oxidized cytochrome b 5 and Met80Ala cyano-cytochrome c

Thermal stability of the [Fe(SCys)4] site in Clostridium pasteurianum rubredoxin: contributions of the local environment and Cys ligand protonation

A new urolith in four cats and a dog: composition and crystal structure

Magnetization studies of the active and fluoride-inhibited derivatives of the reduced catalase of Lactobacillus plantarum: toward a general picture of the anion-inhibited and active forms of the reduced dimanganese catalases

Stability and nickel binding properties of peptides designed as scaffolds for the stabilization of NiII-Fe4S4 bridged assemblies

Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I

New potent agents binding to a poly(dT) sequence in double-stranded DNA: bis(Zn2+-cyclen) and tris(Zn2+-cyclen) complexes

An archaeal b-type cytochrome containing a nonfunctional carbonic anhydrase-like domain

Three high-resolution crystal structures of cadmium-substituted carboxypeptidase A provide insight into the enzymatic function

The nickel enzyme methyl-coenzyme M reductase from methanogenic archaea: In vitro induction of the nickel-based MCR-ox EPR signals from MCR-red2

Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 Å resolution using synchrotron radiation

Iron-sulfur cluster biosynthesis: characterization of Schizosaccharomyces pombe Isa1

Interaction of zinc ions with d(CGCAATTGCG) in a 2.9 Å resolution X-ray structure

Solution structure of cyanoferricytochrome c: ligand-controlled conformational flexibility and electronic structure of the heme moiety

The level of 8-oxo-7,8-dihydro-2′-deoxyguanosine is positively correlated with the size of the labile iron pool in human lymphocytes

Characterization of a metalloregulatory bismuth(III) site in Staphylococcus aureus pI258 CadC repressor

Erratum to: Development of new insulinomimetic zinc(II) picolinate complexes with a Zn(N2O2) coordination mode: structure characterization, in vitro, and in vivo studies

Meetings of interest

SBIC News

JBIC Journal of Biological Inorganic Chemistry : Volume 7, Issue 3, March 2002

JBIC Journal of Biological Inorganic Chemistry : Volume 7, Issue 1-2, January 2002

JBIC Journal of Biological Inorganic Chemistry : Volume 6

JBIC Journal of Biological Inorganic Chemistry : Volume 5

JBIC Journal of Biological Inorganic Chemistry : Volume 4

JBIC Journal of Biological Inorganic Chemistry : Volume 3

JBIC Journal of Biological Inorganic Chemistry : Volume 2

Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I

Content Provider	Springer Nature Link
Author	Antonkine, Mikhail L. Liu, Gaohua Bentrop, Detlef Bryant, Donald A. Bertini, Iva Luchinat, Claudio Golbeck, John H. Stehlik, Dietmar
Copyright Year	2002
Abstract	This work presents the three-dimensional NMR solution structure of recombinant, oxidized, unbound PsaC from Synechococcus sp. PCC 7002. Constraints are derived from homo- and heteronuclear one-, two- and three-dimensional 1H and 15N NMR data. Significant differences are outlined between the unbound PsaC structure presented here and the available X-ray structure of bound PsaC as an integral part of the whole cyanobacterial PS I complex. These differences mainly concern the arrangement of the N- and C-termini with respect to the [4Fe-4S] core domain. In the NMR solution structure of PsaC the C-terminal region assumes a disordered helical conformation, and is clearly different from the extended coil conformation, which is one of the structural elements required to anchor PsaC to the PS I core heterodimer. In solution the N-terminus of PsaC is in contact with the pre-C-terminal region but slides in between the latter and the iron-sulfur core region of the protein. Together, these features result in a concerted movement of the N-terminus and pre-C-terminal region away from the FA binding site, accompanied by a bending of the N-terminus. In comparison, the same terminal regions are positioned much closer to FA and take up an anti-parallel β-sheet arrangement in PsaC bound to PS I. The conformational changes between bound and unbound PsaC correlate with the differences reported earlier for the EPR spectra of reduced FA and FB in bound versus unbound PsaC. The observed different structural features in solution are highly relevant for unraveling the stepwise assembly process of the stromal PsaC, PsaD and PsaE subunits to the PS I core heterodimer. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0321-3.
Starting Page	461
Ending Page	472
Page Count	12
File Format	PDF
ISSN	09498257
Journal	JBIC Journal of Biological Inorganic Chemistry
Volume Number	7
Issue Number	4-5
e-ISSN	14321327
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-01-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biochemistry Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Paramagnetic 1H NMR spectroscopy of the reduced, unbound Photosystem I subunit PsaC: sequence-specific assignment of contact-shifted resonances and identification of mixed-and equal-valence Fe-Fe pairs in [4Fe-4S] centers FA − and FB −

Strains of Synechocystis sp. PCC 6803 with Altered PsaC I. MUTATIONS INCORPORATED IN THE CYSTEINE LIGANDS OF THE TWO [4Fe-4S] CLUSTERS FA AND FB OF PHOTOSYSTEM I

Modified Ligands to FA and FB in Photosystem I II. CHARACTERIZATION OF A MIXED LIGAND [4Fe-4S] CLUSTER IN THE C51D MUTANT OF PsaC UPON REBINDING TO P700-FX CORES

Modified Ligands to FA and FB in Photosystem I PROPOSED CHEMICAL RESCUE OF A [4Fe-4S] CLUSTER WITH AN EXTERNAL THIOLATE IN ALANINE, GLYCINE, AND SERINE MUTANTS OF PsaC

De novo Peptides Modeling the Binding Sites of [4Fe-4S] Clusters in Photosystem I

Assembly of PsaC subunit within stromal ridge of photosystem I core. Comparison of PsaC bound and solution structure

Modified Ligands to FA and Fb in Photosystem I I. STRUCTURAL CONSTRAINTS FOR THE FORMATION OF IRON-SULFUR CLUSTERS IN FREE AND REBOUND PsaC

Solution Structure of the Oxidized 2[4Fe-4S] Ferredoxin from Clostridium Pasteurianum

Crossover of a high-spin (S=7/2, 3/2) to a low-spin (S=1/2) iron-selenium cluster in FA and FB of photosystem I on rebinding of PsaC onto P700-FX cores

Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I

Similar Documents

Paramagnetic 1H NMR spectroscopy of the reduced, unbound Photosystem I subunit PsaC: sequence-specific assignment of contact-shifted resonances and identification of mixed-and equal-valence Fe-Fe pairs in [4Fe-4S] centers FA − and FB −

Strains of Synechocystis sp. PCC 6803 with Altered PsaC I. MUTATIONS INCORPORATED IN THE CYSTEINE LIGANDS OF THE TWO [4Fe-4S] CLUSTERS FA AND FB OF PHOTOSYSTEM I

Modified Ligands to FA and FB in Photosystem I II. CHARACTERIZATION OF A MIXED LIGAND [4Fe-4S] CLUSTER IN THE C51D MUTANT OF PsaC UPON REBINDING TO P700-FX CORES

Modified Ligands to FA and FB in Photosystem I PROPOSED CHEMICAL RESCUE OF A [4Fe-4S] CLUSTER WITH AN EXTERNAL THIOLATE IN ALANINE, GLYCINE, AND SERINE MUTANTS OF PsaC

De novo Peptides Modeling the Binding Sites of [4Fe-4S] Clusters in Photosystem I

Assembly of PsaC subunit within stromal ridge of photosystem I core. Comparison of PsaC bound and solution structure

Modified Ligands to FA and Fb in Photosystem I I. STRUCTURAL CONSTRAINTS FOR THE FORMATION OF IRON-SULFUR CLUSTERS IN FREE AND REBOUND PsaC

Solution Structure of the Oxidized 2[4Fe-4S] Ferredoxin from Clostridium Pasteurianum

Crossover of a high-spin (S=7/2, 3/2) to a low-spin (S=1/2) iron-selenium cluster in FA and FB of photosystem I on rebinding of PsaC onto P700-FX cores

Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: a conformational change occurs upon binding to Photosystem I