NDLI: Effects of extrinsic imidazole ligation on the molecular and electronic structure of cytochrome c

Content Provider	Springer Nature Link
Author	Banci, Lucia Bertini, Iva Liu, Gaohua Lu, Jun Reddig, Tim Tang, Wenxia Wu, Yibing Yao, Yong Zhu, Dexu
Copyright Year	2001
Abstract	Although imidazole ligand binding to cytochrome c is not directly related to its physiological function, it has the potential to provide valuable information on the molecular and electronic structure of the protein. The solution structure of the imidazole adduct of oxidized horse heart cytochrome c (Im-cyt c) has been determined through 2D NMR spectroscopy. The Im-cyt c, 8 mM in 1.2 M imidazole solution at pH 5.7 and 313 K, provided altogether 2542 NOEs (1901 meaningful NOEs) and 194 pseudocontact shifts. The 35 conformers of the family show the RMSD values to the average structure of 0.063±0.007 nm for the backbone and 0.107±0.007 nm for all heavy atoms, respectively. The characterization of Im-cyt c is discussed in detail both in terms of structure and electronic properties. The replacement of the axial ligand Met80 with the exogenous imidazole ligand induces significant conformation changes in both backbone and side chains of the residues located in the distal axial ligand regions. The imidazole ligand binds essentially parallel to the imidazole of the proximal histidine, the two planes forming an angle of 8±7°. The electron delocalization on the heme moiety and the magnetic susceptibility tensor are consistent with these structural features.
Starting Page	628
Ending Page	637
Page Count	10
File Format	PDF
ISSN	09498257
Journal	JBIC Journal of Biological Inorganic Chemistry
Volume Number	6
Issue Number	5-6
e-ISSN	14321327
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-05-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cytochrome c Solution structure Heme protein Biochemistry Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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