NDLI: The uncertainty in the calculation of the amount of blocked and reactive lysine in milk products, as determined by the furosine method

Content Provider	Springer Nature Link
Author	Campos Giménez, Esther Bénet, Thierry Spack, Lionel
Copyright Year	2004
Abstract	The measurement uncertainty in the calculation of the amount of blocked and reactive lysine (as determined by the furosine method) was evaluated according to the procedure described in the Eurachem/CITAC guide. The analytical method involves the chromatographic determination of lysine and furosine after acid hydrolysis. The calculation of blocked and reactive lysine in the initial protein is based on known conversion factors. The estimation of the uncertainty was performed in two steps: (1) determination of the uncertainty in the chromatographic determination of lysine and furosine, and (2) determination of the uncertainty in the calculation of blocked and reactive lysine. The individual contributions to the final uncertainty were identified, quantified, and combined in uncertainty budgets. The largest contribution to the calculation of blocked lysine came from estimating the conversion factor of blocked lysine into furosine during acid hydrolysis. For the calculation of reactive lysine, the main contribution came from the chromatographic determination of lysine. The uncertainty estimates were compared to available validation data (in-house and collaborative standard deviations of reproducibility).
Starting Page	605
Ending Page	614
Page Count	10
File Format	PDF
ISSN	09491775
Journal	Accreditation and Quality Assurance
Volume Number	9
Issue Number	10
e-ISSN	14320517
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-07-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Measurement uncertainty Uncertainty budget Lysine Furosine Amino acids
Content Type	Text
Resource Type	Article
Subject	Chemistry Instrumentation Chemical Engineering Safety, Risk, Reliability and Quality

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