NDLI: Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method

Content Provider	Springer Nature Link
Author	Beljkaš, Bojana Matić, Jovana Milovavić, Ivan Jovav, Pavle Mišan, Aleksandra Šarić, Ljubiša
Copyright Year	2010
Abstract	The objective of this research was to test suitability of the Dumas combustion method to completely substitute the Kjeldahl method in routine laboratory determination of crude protein content in cereals and oilseeds. The validation of the method demonstrated that it is able to determine crude protein content in cereals and oilseeds in an efficient and accurate manner, with a detection limit w(N) = 0.006%, quantification limit w(N) = 0.019%, repeatability precision RSD r = 0.41%, intra-laboratory reproducibility precision RSD R = 0.74%, trueness, expressed in terms of bias b = 0.43%, and linear response between (2.36–19.2) mg N. Measurement uncertainty, expressed as relative expanded uncertainty (coverage factor k = 2, confidence level 95%), was calculated from validation data (U rel = 2.24%). In order to examine the relationship between two methods, 15 cereal grain and oilseed samples were analyzed using Dumas and Kjeldahl procedure. The Kjeldahl procedure gave slightly lower w(N) values than the Dumas procedure: w K(N) = 0.9905 w D(N) = 0.0376 (R 2 = 0.9996). Relative standard deviations and results of homogeneity test obtained during analysis of complex cereal products (cereal breakfast and muesli bars) show that the Dumas combustion method may be less suitable for analysis of such samples compared to Kjeldahl method.
Starting Page	555
Ending Page	561
Page Count	7
File Format	PDF
ISSN	09491775
Journal	Accreditation and Quality Assurance
Volume Number	15
Issue Number	10
e-ISSN	14320517
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-06-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dumas method Kjeldahl method Cereals Oilseeds Validation Measurement uncertainty Marketing Ecotoxicology Biochemistry Analytical Chemistry Commercial Law Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry Instrumentation Chemical Engineering Safety, Risk, Reliability and Quality

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A global approach to method validation and measurement uncertainty

Measurement uncertainty of food carotenoid determination

Validation of the Volhard method for chloride determination in food

Method validation

Estimation of uncertainty for the determination of mercury in food by CVAAS

Comparison of models for estimation of measurement uncertainties. Determination of BOD5 in wastewaters using a manometric method

Calibration uncertainty

Human being as a part of measuring system influencing measurement results

Estimation of measurement uncertainty in food microbiology: The ISO approach

Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method

Similar Documents

A global approach to method validation and measurement uncertainty

Measurement uncertainty of food carotenoid determination

Validation of the Volhard method for chloride determination in food

Method validation

Estimation of uncertainty for the determination of mercury in food by CVAAS

Comparison of models for estimation of measurement uncertainties. Determination of BOD5 in wastewaters using a manometric method

Calibration uncertainty

Human being as a part of measuring system influencing measurement results

Estimation of measurement uncertainty in food microbiology: The ISO approach

Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method