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Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products

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Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products

Content Provider	Springer Nature Link
Author	Grohmann, Lutz Reiting, Ralf Mäde, Dietrich Uhlig, Steffen Simon, Kirsten Frost, Kirstin Randhawa, Gurinder Jit Zur, Katrin
Copyright Year	2015
Abstract	Presence of genetic modifications in rice products originating from China and imported to the European Union market is detected since 2006. Neither these products from China nor any other genetically modified rice lines are approved as food or feed in the EU. The transgenic rice varieties identified contain genetic elements and constructs coding for insect-resistance genes from Bacillus thuringiensis (Bt) coding for insecticidal crystal (cry) proteins. In particular, DNA sequences coding for codon-optimised or fused cry1Ab/c genes and constructs driven by the maize ubiquitin promoter (P-ubiZM1) were identified. For improved screening and identification of genetic modifications present in Asian rice products, two TaqMan-based real-time PCR assays targeting codon-optimised cry1Ab/Ac and the Pubi-cry construct have been developed. These assays have been validated in an international collaborative trial with 17 participants from 10 countries. Based on a new mathematical–statistical model and an adjusted experimental set-up of the collaborative trial, a close examination of the limit of detection (LOD 95%) and the probability of detection of the qualitative PCR assays was conducted. The evaluation of the method performance characteristics and results of the collaborative trial validation are presented.
Starting Page	85
Ending Page	96
Page Count	12
File Format	PDF
ISSN	09491775
Journal	Accreditation and Quality Assurance
Volume Number	20
Issue Number	2
e-ISSN	14320517
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-03-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genetically modified organism (GMO) Cry1Ab Maize ubiquitin promoter (P-ubiZM1) Rice Detection method Real-time PCR Probability of detection (POD) Collaborative trial study Analytical Chemistry Food Science Biochemistry Commercial Law Ecotoxicology Marketing
Content Type	Text
Resource Type	Article
Subject	Chemistry Instrumentation Chemical Engineering Safety, Risk, Reliability and Quality

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