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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mussell, Chris Wolff Briche, Céline S. J. Hopley, Chris O’Conr, Gavin |
| Copyright Year | 2007 |
| Abstract | The method used at LGC for analysis of “total” 19-norandrosterone (19-norandrosterone glucuronide plus “free” 19-norandrosterone) in urine for the Comité Consultatif pour la Quantité de Matière Pilot Study (CCQM-P68) is described. The analytical method used was a modified version of the method developed at the National Measurement Institute of Australia, which used a hydrolysis and derivatisation procedure first described by the German Sports University. This method is routinely used by World Anti-Doping Agency-accredited laboratories for sports drug testing. The main modifications made to the method were the use of 19-norandrosterone glucuronide as a calibration standard and 19-norandrosterone glucuronide-d4 as an isotopically labelled internal standard, and the use of a bench-top quadrupole gas chromatograph–mass spectrometer. The results produced by LGC (2.14 ± 0.15 ng g−1 expanded uncertainty, coverage factor k = 2) were in excellent agreement with those from other participating national metrology institutes and thus further validates the exact-matching isotope-dilution mass spectrometric procedures used at LGC for a wide range of reference measurement applications, including measurement of ng g−1 levels of steroids in a biological matrix. |
| Starting Page | 469 |
| Ending Page | 474 |
| Page Count | 6 |
| File Format | |
| ISSN | 09491775 |
| Journal | Accreditation and Quality Assurance |
| Volume Number | 12 |
| Issue Number | 9 |
| e-ISSN | 14320517 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2007-06-16 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Isotope-dilution mass spectrometry Nandrolone in urine Norandrosterone Norandrosterone glucuronide Reference method Steroids Marketing Ecotoxicology Commercial Law Biochemistry Food Science Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Instrumentation Chemical Engineering Safety, Risk, Reliability and Quality |
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