NDLI: Abomasal infusion of arginine stimulates SCD and C/EBPß gene expression, and decreases CPT1ß gene expression in bovine adipose tissue independent of conjugated linoleic acid

Content Provider	Springer Nature Link
Author	Choi, Seong Ho Wickersham, Tryon A. Wu, Guoyao Gilmore, L. Anne Edwards, Holly D. Park, Sung Kwon Kim, Kyoung Hoon Smith, Stephen B.
Copyright Year	2013
Abstract	Based on previous research with bovine peadipocytes, we hypothesized that infusion of arginine into the abomasum of Angus steers stimulates stearoyl-CoA desaturase (SCD) gene expression in bovine subcutaneous (s.c.) adipose tissue, and that this would be attenuated by conjugated linoleic acid (CLA). Growing Angus steers were infused abomasally with l-arginine 50 g/day; n = 13; provided as l-arginine HCl) or l-alanine (isonitrogenous control, 100 g/day; n = 11) for 14 days. For the subsequent 14 days, half of the steers in each amino acid group were infused with CLA (100 g/day). Body weight gain and average daily gain were unaffected (P > 0.15) by infusion of arginine or CLA into the abomasum. The plasma concentrations of cis-9, trans-11 and trans-10, cis-12 CLA were increased CLA infusion (P = 0.001) and infusion of arginine increased plasma arginine (P = 0.01). Compared with day 0, fatty acid synthase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase enzyme activities in s.c. adipose tissue increased by day 14 in steers infused with either alanine or arginine (all P < 0.01). NADP-MDH activity was higher (P = 0.01) in steers infused with arginine than in steers infused with arginine plus CLA by day 28, but lipid synthesis in vitro from glucose and acetate was unaffected by infusion of either arginine or CLA (P > 0.40). By day 28, C/EBPβ and SCD gene expression was higher, and CPT1β gene expression was lower, in s.c. adipose tissue of steers infused with arginine than in steers infused with alanine (±CLA) (P = 0.05). CLA decreased adipose tissue oleic acid (18:1n-9) in alanine- or arginine-infused steers (P = 0.05), although CLA had no effect on SCD gene expression. The data indicate that supplemental arginine promotes adipogenic gene expression and may promote lipid accumulation in bovine adipose tissue. l-Arginine may beneficially improve beef quality for human consumption.
Starting Page	353
Ending Page	366
Page Count	14
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	46
Issue Number	2
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2013-12-11
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Adipose tissue Adipogenesis Arginine Beef cattle Conjugated linoleic acid Biochemistry Analytical Chemistry Biochemical Engineering Life Sciences Proteomics Neurobiology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Lipid metabolism in pigs fed supplemental conjugated linoleic acid and/or dietary arginine

Supplementary Linseed during the Late Fattening Period Increases Oleic Acid, Conjugated Linoleic Acid, and PPAR Gamma Gene Expression, and Decreases SCD Expression in Intramuscular Adipose Tissue from Yanbian Yellow Cattle

Feeding conjugated linoleic acids (cla) decreases lipogenesis and alters expression of lipogenic genes in porcine adipose tissue (2000).

High fat feeding and dietary l-arginine supplementation differentially regulate gene expression in rat white adipose tissue

Arginine nutrition and fetal brown adipose tissue development in nutrient-restricted sheep

Arginine nutrition and fetal brown adipose tissue development in diet-induced obese sheep

9cis,11trans conjugated linoleic acid (CLA) is synthesised and desaturated into conjugated 18:3 in bovine adipose tissues

The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue

Daily intake of conjugated linoleic acid-enriched yoghurts: effects on energy metabolism and adipose tissue gene expression in healthy subjects

Abomasal infusion of arginine stimulates SCD and C/EBPß gene expression, and decreases CPT1ß gene expression in bovine adipose tissue independent of conjugated linoleic acid

Similar Documents

Lipid metabolism in pigs fed supplemental conjugated linoleic acid and/or dietary arginine

Supplementary Linseed during the Late Fattening Period Increases Oleic Acid, Conjugated Linoleic Acid, and PPAR Gamma Gene Expression, and Decreases SCD Expression in Intramuscular Adipose Tissue from Yanbian Yellow Cattle

Feeding conjugated linoleic acids (cla) decreases lipogenesis and alters expression of lipogenic genes in porcine adipose tissue (2000).

High fat feeding and dietary l-arginine supplementation differentially regulate gene expression in rat white adipose tissue

Arginine nutrition and fetal brown adipose tissue development in nutrient-restricted sheep

Arginine nutrition and fetal brown adipose tissue development in diet-induced obese sheep

9cis,11trans conjugated linoleic acid (CLA) is synthesised and desaturated into conjugated 18:3 in bovine adipose tissues

The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue

Daily intake of conjugated linoleic acid-enriched yoghurts: effects on energy metabolism and adipose tissue gene expression in healthy subjects

Abomasal infusion of arginine stimulates SCD and C/EBPß gene expression, and decreases CPT1ß gene expression in bovine adipose tissue independent of conjugated linoleic acid