NDLI: In vitro and in vivo evaluation of BMAP-derived peptides for the treatment of cystic fibrosis-related pulmonary infections

Content Provider	Springer Nature Link
Author	Mardirossian, Mario Pompilio, Arianna Crocetta, Valentina Nicola, Serena Guida, Filomena Degasperi, Margherita Gennaro, Renato Di Bonaventura, Giovanni Scocchi, Marco
Copyright Year	2016
Abstract	Patients with cystic fibrosis require pharmacological treatment against chronic lung infections. The alpha-helical antimicrobial peptides BMAP-27 and BMAP-28 have shown to be highly active in vitro against planktonic and sessile forms of multidrug-resistant Pseudomonas aeruginosa, Staphylococcus aureus, and Stenotrophomonas maltophilia cystic fibrosis strains. To develop small antibacterial peptides for therapeutic use, we tested shortened/modified BMAP fragments, and selected the one with the highest in vitro antibacterial activity and lowest in vivo acute pulmonary toxicity. All the new peptides have shown to roughly maintain their antibacterial activity in vitro. The 1–18 N-terminal fragment of BMAP-27, showing MIC90 of 16 µg/ml against P. aeruginosa isolates and strain-dependent anti-biofilm effects, showed the lowest pulmonary toxicity in mice. However, when tested in a murine model of acute lung infection by P. aeruginosa, BMAP-27(1–18) did not show any curative effect. If exposed to murine broncho-alveolar lavage fluid BMAP-27(1–18) was degraded within 10 min, suggesting it is not stable in pulmonary environment, probably due to murine proteases. Our results indicate that shortened BMAP peptides could represent a starting point for antibacterial drugs, but they also indicate that they need a further optimization for effective in vivo use.
Starting Page	2253
Ending Page	2260
Page Count	8
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	48
Issue Number	9
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2016-06-06
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Antimicrobial peptide Cathelicidin BMAP Cystic fibrosis Biofilm Multidrug-resistance In vivo degradation Biochemistry Analytical Chemistry Biochemical Engineering Life Sciences Proteomics Neurobiology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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