NDLI: An isotope-dilution, GC–MS assay for formate and its application to human and animal metabolism

Content Provider	Springer Nature Link
Author	Lamarre, Simon G. MacMillan, Luke Morrow, Gregory P. Randell, Edward Pongpparat, Theerawat Brosnan, Margaret E. Brosnan, John T.
Copyright Year	2014
Abstract	Formate, a crucial component of one-carbon metabolism, is increasingly recognized as an important intermediate in production and transport of one-carbon units. Unlike tetrahydrofolate-linked intermediates, it is not restricted to the intracellular milieu so that circulating levels of formate can provide insight into cellular events. We report a novel isotope-dilution, GC–MS assay employing derivatization by 2,3,4,5,6-pentafluorobenzyl bromide for the determination of formate in biological samples. This assay is robust and sensitive; it may be applied to the measurement of formate in serum, plasma and urine. We demonstrate how this method may be applied by providing the first characterization of formate levels in a human population; formate levels were higher in males than in females. We also show how this procedure may be applied for the measurement of in vivo kinetics of endogenous formate production in experimental animals.
Starting Page	1885
Ending Page	1891
Page Count	7
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	46
Issue Number	8
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2014-04-10
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Serine Glycine One-carbon metabolism Folate Methanol Biochemistry Analytical Chemistry Biochemical Engineering Life Sciences Proteomics Neurobiology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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