NDLI: Connective tissue growth factor is a downstream mediator for preptin-induced proliferation and differentiation in human osteoblasts

Content Provider	Springer Nature Link
Author	Liu, You Shuo Lu, Ying Liu, Wei Xie, Hui Luo, Xiang Hang Wu, Xian Ping Yuan, Ling Qing Liao, Er Yuan
Copyright Year	2009
Abstract	Preptin, a newly isolated 34-amino-acid peptide hormone that is cosecreted with insulin and amylin from pancreatic beta-cells, has emerged as a regulatory element in bone metabolism, but its mechanism remains unclear. We assessed the effects of preptin on proliferation and differentiation of human osteoblasts and investigated the mechanism involved. Our results demonstrated that preptin promoted human osteoblasts proliferation and alkaline phosphatase activity. Suppression of connective tissue growth factor (CTGF), which was upregulated by preptin in a dose- and time-dependent manner, with small interfering RNA (siRNA) abolished the preptin-induced human osteoblasts proliferation and differentiation. Preptin induced activation of ERK mitogen-activated protein kinase (MAPK), but not p38 or JNK in human osteoblasts. Furthermore, pretreatment of human osteoblasts with the ERK inhibitor PD98059 abolished the preptin-induced CTGF secretion and blocked the promoting effect of preptin on osteoblasts proliferation and differentiation. These data demonstrated that preptin is involved in bone anabolism mediated by ERK/CTGF in human osteoblasts and may contribute to the preservation of bone mass observed in hyperinsulinemic states, such as obesity.
Starting Page	763
Ending Page	769
Page Count	7
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	38
Issue Number	3
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2009-03-31
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Preptin Connective tissue growth factor Osteoblasts Extracellular signal regulated kinase Neurobiology Proteomics Life Sciences Biochemical Engineering Analytical Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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Connective tissue growth factor is a downstream mediator for preptin-induced proliferation and differentiation in human osteoblasts

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Connective tissue growth factor is a downstream mediator for preptin-induced proliferation and differentiation in human osteoblasts