NDLI: Expression of transglutaminase-2 isoforms in normal human tissues and cancer cell lines: dysregulation of alternative splicing in cancer

Content Provider	Springer Nature Link
Author	Phatak, V. M. Croft, S. M. Rameshaiah Setty, S. G. Scarpellini, A. Hughes, D. C. Rees, R. McArdle, S. Verderio, E. A. M.
Copyright Year	2011
Abstract	The multiple enzymatic activities and functions of transglutaminase type 2 (TG2) may be attributed to alternative TG2 molecules produced by differential splicing of TG2 mRNA. Different RNA transcripts of the human TG2 gene (TGM2) have been identified, but the expression of TG2 multiple transcripts has never been systematically addressed. We have confirmed and rationalized the main TG2 variants and developed a screening assay for the detection of alternative splicing of TG2, based on real-time reverse-transcription PCR. We have quantified the multiple TG2 transcripts in a wide range of normal tissues and in cancer cell lines from four different sites of origin. Our data show a significant correlation in the expression of canonical and alternative TG2 isoforms in normal human tissue, but differences in alternative splicing of TG2 in cancer cell lines, suggesting that in cancer cells the alternative splicing of TG2 is a more active process.
Starting Page	33
Ending Page	44
Page Count	12
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	44
Issue Number	1
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2011-11-17
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Transglutaminase-2 Alternative splicing Splicing dysregulation Normal tissue Cancer Cell lines Biochemistry Analytical Chemistry Biochemical Engineering Life Sciences Proteomics Neurobiology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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