NDLI: Erythrocyte glutathione transferase: a potential new biomarker in chronic kidney diseases which correlates with plasma homocysteine

Content Provider	Springer Nature Link
Author	Dessì, Mariarita ce, Annalisa Dawood, Kutayba F. Galli, Francesco Taccone Gallucci, Massimo Fabrini, Raffaele Bocedi, Alessio Massoud, Renato Fucci, Giorgio Pastore, Anna Manca di Villahermosa, Simone Zingaretti, Viviana Federici, Giorgio Ricci, Giorgio
Copyright Year	2011
Abstract	The erythrocyte glutathione S-transferase (e-GST) is a member of a superfamily of inducible enzymes involved in cell detoxification that shows an increased expression in chronic kidney disease (CKD) patients. We propose a new automated analysis procedure for e-GST activity that has been validated in 72 CKD patients and 62 maintenance hemodialysis patients (MHD). Regression analysis was carried out to assess association between e-GST activity data, main clinical variables, and plasma homocysteine (Hcy), a modified sulfur amino acid known as potential risk factor for cardiovascular disease that is increased above normal levels in more than 90% of the uremic patients. An increased e-GST activity was confirmed in MHD patients (N = 62; 10.2 ± 0.4 U/gHb) compared with healthy subjects (N = 80; 5.8 ± 0.4 U/gHb), and as an original finding, a significant increase of e-GST activity was observed in pre-dialysis CKD patients with a positive correlation with disease severity weighted according to the four stages of “Kidney Disease Outcomes Quality Initiative” classification (7.4 ± 0.5, 8 ± 1, 9.5 ± 0.6, 12 ± 1 U/gHb, respectively). No correlation was found between e-GST activity and hemoglobin, transferrin, blood iron and the markers of systemic inflammation and renal function such as alpha-1 acid glycoprotein and high-sensitive C-Reactive Protein, beta-2 microglobulin and the index of malnutrition-inflammation PINI, while a significant correlation was observed for the first time between plasma Hcy and e-GST activity (r 2 = 0.64, P < 0.0001) in MHD patients. Hcy, however, was not identified as an inhibitor of e-GST enzyme. The results in this study suggest the potential for automated e-GST analysis as a valuable tool to further explore phase II-related uremic toxicity in CKD and MHD patients.
Starting Page	347
Ending Page	354
Page Count	8
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	43
Issue Number	1
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2011-10-08
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Chronic kidney disease Erythrocyte glutathione transferase Hyperhomocysteinemia Maintenance hemodialysis Life Sciences Proteomics Biochemical Engineering Biochemistry Neurobiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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