NDLI: A new metabolic route for the production of gamma-aminobutyric acid by Corynebacterium glutamicum from glucose

Content Provider	Springer Nature Link
Author	Jorge, João M. P. Leggewie, Christian Wendisch, Volker F.
Copyright Year	2016
Abstract	Gamma-aminobutyric acid (GABA), a non-protein amino acid widespread in nature, is a component of pharmaceuticals, foods, and the biodegradable plastic polyamide 4. Corynebacterium glutamicum shows great potential for the production of GABA from glucose. GABA added to the growth medium hardly affected growth of C. glutamicum, since a half-inhibitory concentration of 1.1 M GABA was determined. As alternative to GABA production by glutamate decarboxylation, a new route for the production of GABA via putrescine was established in C. glutamicum. A putrescine-producing recombinant C. glutamicum strain was converted into a GABA producing strain by heterologous expression of putrescine transaminase (PatA) and gamma-aminobutyraldehyde dehydrogenase (PatD) genes from Escherichia coli. The resultant strain produced 5.3 ± 0.1 g L−1 of GABA. GABA production was improved further by adjusting the concentration of nitrogen in the culture medium, by avoiding the formation of the by-product N-acetylputrescine and by deletion of the genes for GABA catabolism and GABA re-uptake. GABA accumulation by this strain was increased by 51 % to 8.0 ± 0.3 g L−1, and the volumetric productivity was increased to 0.31 g L−1 h−1; the highest volumetric productivity reported so far for fermentative production of GABA from glucose in shake flasks was achieved.
Starting Page	2519
Ending Page	2531
Page Count	13
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	48
Issue Number	11
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2016-06-11
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Gamma-aminobutyric acid Putrescine Corynebacterium glutamicum Putrescine transaminase Gamma-aminobutyraldehyde dehydrogenase Gamma-aminobutyrate aminotransferase, GABA Biochemistry Analytical Chemistry Biochemical Engineering Life Sciences Proteomics Neurobiology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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