NDLI: A first partial Aplysia californica proteome

Content Provider	Springer Nature Link
Author	Sun, Yanwei Monje, Francisco J. Pollak, Daniela D. Lubec, Gert
Copyright Year	2010
Abstract	Aplysia proteins have not been studied systematically and it was therefore the aim of the study to carry out protein profiling in ganglia from Aplysia californica (AC). AC ganglia were extirpated, proteins extracted and run on 2DE with subsequent in-gel digestion, followed by identification of proteins by nano-LC–ESI–MS/MS on an ion trap. Proteins were identified based upon a public Aplysia EST database. Out of 408 picked spots, 276 spots were identified corresponding to 172 ESTs and 118 individual proteins. The range of sequence coverage was between 14 and 80% and the average amount of peptides used for the identification of proteins was 9 (from 3 to 24). Mean score for protein identification was 516. Comparison of protein levels between cerebral, pleural, pedal and abdominal ganglia revealed a series of significant differences including: signaling, metabolism, cytoskeleton and structural, redox, chaperone, replication/transcription and electron/proton transport proteins. The generation of a protein map complements transcriptional studies carried out in AC ganglia. The findings provide the basis for investigation into post-translational modifications, splice variants and assist in the generation of antibodies against AC proteins. Moreover, differences in protein expression between ganglia may be valuable for the design of future studies in neurobiology of AC.
Starting Page	955
Ending Page	968
Page Count	14
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	41
Issue Number	4
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2010-11-11
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	2-DE Aplysia californica Nano-LC–ESI–MS/MS Protein map Protein profiling Ganglia Life Sciences Neurobiology Biochemical Engineering Proteomics Analytical Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterisation of the thiol–disulphide chemistry of desmopressin by LC, μ-LC, LC-ESI-MS and Maldi-Tof

Nano-structured support materials, their characterisation and serum protein profiling through MALDI/TOF-MS

Chemically modified diamond-like carbon (DLC) for protein enrichment and profiling by MALDI-MS

LC/MS evaluation of metabolism and membrane transport of bombesin peptides

Hypotaurine disproportionation reaction: Identification of products

Erratum to: LC/MS evaluation of metabolism and membrane transport of bombesin peptides

“Brainprot” – generating the proteome of the brain

Peptaibiomics: an advanced, rapid and selective analysis of peptaibiotics/peptaibols by SPE/LC-ES-MS

Activity-based proteomics: enzymatic activity profiling in complex proteomes

A first partial Aplysia californica proteome

Similar Documents

Characterisation of the thiol–disulphide chemistry of desmopressin by LC, μ-LC, LC-ESI-MS and Maldi-Tof

Nano-structured support materials, their characterisation and serum protein profiling through MALDI/TOF-MS

Chemically modified diamond-like carbon (DLC) for protein enrichment and profiling by MALDI-MS

LC/MS evaluation of metabolism and membrane transport of bombesin peptides

Hypotaurine disproportionation reaction: Identification of products

Erratum to: LC/MS evaluation of metabolism and membrane transport of bombesin peptides

“Brainprot” – generating the proteome of the brain

Peptaibiomics: an advanced, rapid and selective analysis of peptaibiotics/peptaibols by SPE/LC-ES-MS

Activity-based proteomics: enzymatic activity profiling in complex proteomes

A first partial Aplysia californica proteome