NDLI: Plasmalemma-associated malate dehydrogenase activity in onion root cells

Content Provider	Springer Nature Link
Author	Córdoba Pedregosa, M. C. González Reyes, J. A. Serra, A. Villalba, J. M. Navas, P. Córdoba, F.
Copyright Year	1998
Abstract	Plasma membrane vesicles isolated from onion roots showed oxaloacetate reductase activity as well as other oxidoreductase activities. Purification and further sequencing showed that the protein responsible for the activity is a 40 kDa protein which corresponds to the cytosolic soluble malate dehydrogenase. However, the activity remained bound to the membrane after repeated freezing and thawing cycles and further washing, excluding a cytosolic contamination as the source of the activity. Furthermore, a second 28 kDa protein has been copurified together with the 40 kDa protein. The plasmalemma oxaloacetate reductase activity shows both donor and acceptor sites located towards the cytoplasmic side of the plasma membrane. This enzyme catalyzed the oxidation of NADH by oxaloacetate and the reduction of NAD+ by malate in the presence of an oxaloacetate-withdrawing system. We conclude that a significant amount of the cytosolic malate dehydrogenase can be specifically attached to the cytosolic face of the plasmalemma. A possible role in a putative malate shuttle associated to the plasma membrane is discussed.
Starting Page	29
Ending Page	36
Page Count	8
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	205
Issue Number	1-4
e-ISSN	16156102
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-01-01
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Allium cepa L. Malate dehydrogenase root Plasma membrane Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

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