NDLI: Biocompatibility of different nanostructured TiO2 scaffolds and their potential for urologic applications

Content Provider	Springer Nature Link
Author	Imani, Roghayeh Pazoki, Meysam Zupančič, Daša Kreft, Mateja Erdani Kralj Iglič, Veronika Veranič, Peter Iglič, Aleš
Copyright Year	2015
Abstract	Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient’s quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of clinical applications and are already widely used in orthopedic or dental medicine. The current study was conducted to synthesize TiO2 nanotubes by the anodization method and TiO2 nanowires and nanospheres by the chemical vapor deposition method. These scaffolds were characterized with scanning electron microscopy (SEM) and X-ray diffraction (XRD) methods. In order to test the urologic applicability of generated TiO2 scaffolds, we seeded the normal porcine urothelial (NPU) cells on TiO2 nanotubes, TiO2 nanowires, TiO2 nanospheres, and on the standard porous membrane. The viability and growth of the cells were monitored everyday, and after 3 weeks of culturing, the analysis with scanning electron microscope (SEM) was performed. Our results showed that the NPU cells were attached on all scaffolds; they were viable and formed a multilayered epithelium, i.e., urothelium. The apical plasma membrane of the majority of superficial NPU cells, grown on all three different TiO2 scaffolds and on the porous membrane, exhibited microvilli; thus, indicating that they were at a similar differentiation stage. The maximal caliper diameter measurements of superficial NPU cells revealed significant alterations, with the largest cells being observed on nanowires and the smallest ones on the porous membrane. Our findings indicate that different nanostructured TiO2 scaffolds, especially nanowires, have a great potential for tissue engineering and should be further investigated for various urologic applications.
Starting Page	1439
Ending Page	1447
Page Count	9
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	253
Issue Number	6
e-ISSN	16156102
Language	English
Publisher	Springer Vienna
Publisher Date	2015-10-24
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanostructured TiO2 scaffolds Anodization Chemical vapor deposition Normal porcine urothelial cells Urologic application Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

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