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| Content Provider | Springer Nature Link |
|---|---|
| Author | Custers, J. B. M. Snepvangers, S. C. H. J. Jansen, H. J. Zhang, L. Lookeren Campagne, M. M. |
| Copyright Year | 1999 |
| Abstract | The cauliflower mosaic virus 35S (35S-CaMV) promoter, which is generally used as a constitutive promoter in plants, is known to be silent during microspore and pollen development. Here we analyzed whether the 35S-CaMV promoter fused to thegus (β-glucuronidase) gene can be used as a marker for early sporophytic development in embryogenic microspore cultures of tobacco andBrassica napus. In microspore culture ofB. napus, the 35S-CaMV promoter remained off from the start of embryogenic culture up to the mid-cotyledonary embryo stage. 35S-CaMV promoter activity was only present in those microspores that initiated sporophytic development, but failed to enter embryogenic development. Similar results were also obtained with shed-microspore cultures of tobacco, in which rapid, direct embryogenesis takes place. In isolated-microspore cultures, in which embryogenesis is delayed, an intermitting period of sporophytic development was observed, characterized by extensive 35S-CaMV promoter activity. Therefore, the 35S-CaMV promoter discriminates between two classes of sporophytic development: it is activated in microspores which change fate from gametophytic into (temporarily) nonembryogenic sporophytic development, whereas the promoter is silent in sporophytic microspores that enter embryogenic development directly. This mirrors our observation that the 35S-CaMV promoter is also silent in young zygotic embryos. |
| Starting Page | 257 |
| Ending Page | 264 |
| Page Count | 8 |
| File Format | |
| ISSN | 0033183X |
| Journal | Protoplasma |
| Volume Number | 208 |
| Issue Number | 1-4 |
| e-ISSN | 16156102 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 1999-01-01 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Brassica napus Microspore embryogenesis Cauliflower mosaic virus 35S promoter Sporophytic development Tobacco Zygotic embryogenesis Cell Biology Plant Sciences Zoology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science Medicine |
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