NDLI: Caffeine inhibition of cytokinesis: effect on the phragmoplast cytoskeleton in livingTradescantia stamen hair cells

Content Provider	Springer Nature Link
Author	Valster, A. H. Hepler, P. K.
Copyright Year	1997
Abstract	The distribution of microtubules and actin microfilaments during caffeine-induced inhibition of cell plate formation has been studied in livingTradescantia stamen hair cells. Previous studies have shown that caffeine allows cell plate initiation but prevents its completion, resulting in binucleate cells. In the present study, confocal microscopy of cells microinjected with fluorescent brain tubulin or phalloidin, and cultured in the presence 5 mM caffeine, revealed that the initiation and early lateral expansion phase of the phragmoplast occur normally. However, caffeine completely inhibits the formation of the cytoskeletal torus which occurs in untreated cells during the late stages of cell plate and phragmoplast expansion. Caffeine further causes the disintegration of the incomplete cell plate. The results allow us to distinguish two phases in cell plate and phragmoplast growth: the initiation and early expansion phase, which is not affected by caffeine, and the late lateral expansion phase, which is completely inhibited in the presence of caffeine. Also in this study, the use of a high phalloidin concentration has revealed structural detail about the actin microfilaments involved in cell plate formation: microfilaments are observed that link the expanding edge of the phragmoplast with the cortical division site. In addition, cortical actin patches are observed within the actin depleted zone that might play a role in guidance of phragmoplast and cell plate expansion.
Starting Page	155
Ending Page	166
Page Count	12
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	196
Issue Number	3-4
e-ISSN	16156102
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-01-01
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Caffeine Cytokinesis Cytoskeleton Microinjection Phragmoplast Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Accessory phragmoplast corrects abnormal cytokinesis in wheat x rye hybrids

Stages of formation of mobile phragmoplast in meiosis with successive cytokinesis

Processes providing the phragmoplast centrifugal movement in cereal meiosis

ROS homeostasis as a prerequisite for the accomplishment of plant cytokinesis

Plasma membrane-cell wall connections: Roles in mitosis and cytokinesis revealed by plasmolysis ofTradescantia virginiana leaf epidermal cells

Microtubule and actin filament organization during acentral divisions in potato suspension culture cells

The cytoskeleton and spatial control of cytokinesis in the plant life cycle

Calmodulin is uniformly distributed during cell division in living stamen hair cells ofTradescantia virginiana

Nuclear localization of profilin during the cell cycle in Tradescantia virginiana stamen hair cells

Caffeine inhibition of cytokinesis: effect on the phragmoplast cytoskeleton in livingTradescantia stamen hair cells

Similar Documents

Accessory phragmoplast corrects abnormal cytokinesis in wheat x rye hybrids

Stages of formation of mobile phragmoplast in meiosis with successive cytokinesis

Processes providing the phragmoplast centrifugal movement in cereal meiosis

ROS homeostasis as a prerequisite for the accomplishment of plant cytokinesis

Plasma membrane-cell wall connections: Roles in mitosis and cytokinesis revealed by plasmolysis ofTradescantia virginiana leaf epidermal cells

Microtubule and actin filament organization during acentral divisions in potato suspension culture cells

The cytoskeleton and spatial control of cytokinesis in the plant life cycle

Calmodulin is uniformly distributed during cell division in living stamen hair cells ofTradescantia virginiana

Nuclear localization of profilin during the cell cycle in Tradescantia virginiana stamen hair cells

Caffeine inhibition of cytokinesis: effect on the phragmoplast cytoskeleton in livingTradescantia stamen hair cells