NDLI: Suppression of lytic replication of Kaposi’s sarcoma-associated herpesvirus by autophagy during initial infection in NIH 3T3 fibroblasts

Content Provider	Springer Nature Link
Author	Jang, Gun Hee Lee, Jihui Kim, Na Yeon Kim, Jae Hyeon Yeh, Jung Yong Han, Minsub Ahn, Soon Kil Kang, Hara Lee, Michael
Copyright Year	2015
Abstract	Kaposi’s sarcoma-associated herpesvirus (KSHV) is the infectious cause of the angioproliferative neoplasm Kaposi’s sarcoma (KS). We first confirmed the susceptibility of NIH 3T3 fibroblasts to KSHV by infecting them with BCP-1-derived KSHV. Lytic replication of KSHV was confirmed by PCR amplification of viral DNA isolated from culture supernatants of KSHV-infected cells. The template from KSHV-infected NIH 3T3 cells resulted in an intense viral DNA PCR product. A time course experiment revealed the disappearance of KSHV-specific DNA in culture supernatant of NIH 3T3 cells during a period between 48 h and 72 h postinfection. Furthermore, 3 days postinfection, infected NIH 3T3 cells showed no evidence of latent or lytic transcripts, including LANA, vFLIP, vCyclin, and vIL-6. These results imply that KSHV infection in NIH 3T3 cells is unstable and is rapidly lost on subsequent culturing. Additionally, we detected an enhancement of autophagy early in infection with KSHV. More interestingly, inhibition of autophagy by Beclin 1 siRNA or 3-methyladenine significantly increased the amount of KSHV-specific DNA in the culture supernatant of NIH 3T3 cells when compared to the group treated with KSHV infection alone, implying that autophagy prevents lytic replication of KSHV. Taken together, our data suggest that autophagy could be one of the cellular mechanisms utilized by host cells to promote viral clearance.
Starting Page	595
Ending Page	604
Page Count	10
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	161
Issue Number	3
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2015-11-30
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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