NDLI: Characterization of a new phage, termed ϕA318, which is specific for Vibrio alginolyticus

Content Provider	Springer Nature Link
Author	Lin, Ying Rong Chiu, Chi Wen Chang, Feng Yi Lin, Chan Shing
Copyright Year	2012
Abstract	Vibrio alginolyticus is an opportunistic pathogen of animals and humans; its related strains can also produce tetrodotoxin and hemolysins. A new phage, ϕA318, which lysed its host V. alginolyticus with high efficiency, was characterized. The burst size of ϕA318 in V. alginolyticus was 72 PFU/bacterium at an MOI of 1 at room temperature; the plaque size was as large as 5 mm in diameter. Electron microscopy (EM) of the phage particles revealed a 50- to 55-nm isomorphous icosahedral head with a 12-nm non-contractile tail, similar to the T7-like phages of the family Podoviridae. Phylogenetic analysis based on complete sequences of the DNA-directed RNA polymerase gene revealed that ϕA318 had 28-47% amino acid identity to enterobacteria phages T7 and SP6, and other Vibrio phages, and the phylogenetic distance suggested that ϕA318 could be classified as a new T7-like bacteriophage. Nevertheless, several motifs in the ϕA318 phage RNA polymerase were highly conserved, including DFRGR (T7-421 motif), DG (T7-537 motif), PSEKPQDIYGAVS (T7-563 motif), RSMTKKPVMTL PYGS (T7-627 motif), and HDS (T7-811 motif). Genetic analysis indicated that phage ϕA318 is not a thermostable direct hemolysin producer. The results suggest that the MOI should be higher than 0.1 to prevent the chance of hemolysin production by the bacteria before they are lysed by the phage.
Starting Page	917
Ending Page	926
Page Count	10
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	157
Issue Number	5
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2012-02-11
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Virology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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