NDLI: Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus

Content Provider	Springer Nature Link
Author	Wu, Pei Ru Chien, Wan Chu Okuda, Mitsuru Takeshita, Miru Yeh, Shyi Dong Wang, Yun Chi Chen, Tsung Chi
Copyright Year	2014
Abstract	Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related.
Starting Page	529
Ending Page	536
Page Count	8
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	160
Issue Number	2
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2014-11-27
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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