NDLI: Genome evolution of tobacco mosaic virus populations during long-term passaging in a diverse range of hosts

Content Provider	Springer Nature Link
Author	Kearney, C. M. Thomson, M. J. Roland, K. E.
Copyright Year	1999
Abstract	The effects of host changes on plant virus genome evolution was studied by nucleotide sequencing. A single tobacco (Nicotiana tabacum cv. Xanthi) plant was inoculated with in vitro transcripts from a plasmid clone of tobacco mosaic tobamovirus (TMV). This initial viral population was then transferred 11–12 times in parallel populations in 7 plant host species (1–4 replicates each) over a period of 413–515 days. Virion RNA was then isolated, reverse transcribed, amplified, cloned in bacteria, and sequenced. Portions of the coat protein, movement protein, and replicase genes were sequenced. Fourteen unique mutations were detected from a total of 188 clones (35,607 bases) sequenced, indicating a relatively small overall mutation rate of 3.1 × 10−4 nucleotide substitutions/base-year. A small Ka/Ks value of 0.09 was also found, indicating selection against amino acid changes. Eighty-five percent of the substitutions were transitions. A G’ST value of 0.7 for the coat protein gene suggested that host type affected sequence changes in this region of the genome, but χ2 analysis did not support this conclusion. This is the first study using sequencing to compare representative sample sections of a plant viral genome following a major selective disturbance such as extended passaging in an alternate host.
Starting Page	1513
Ending Page	1526
Page Count	14
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	144
Issue Number	8
e-ISSN	14328798
Language	English
Publisher	Springer-Verlag
Publisher Date	2014-05-21
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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