NDLI: Potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency

Content Provider	Springer Nature Link
Author	Flasinski, S. Cassidy, B. G.
Copyright Year	1998
Abstract	Aphid transmission of potyviruses depends on the presence of specific sequence domains in two virus encoded proteins, the coat protein (CP) and helper component-proteinase (HC-Pro). Aphid transmissable peanut stripe virus (PStV), like most potyviruses, has an Asp-Ala-Gly (DAG) motif in the amino-terminal part of the CP. Peanut Mottle Virus (PeMoV) was determined to be highly aphid transmissible but has a unique Asp-Ala-Ala-Ala (DAAA) motif. To determine if the DAAA motif could functionally replace the DAG motif in PStV, mutations were made in a full-length cDNA clone of PStV. All of the mutations in the CP DAG motif abolished aphid transmissibility of PStV but did not affect virus infectivity. The aphid transmissibility of the PStV-DAAA mutant was partially restored by feeding aphids an artificial diet containing puri- fied virus and PeMoV HC-Pro. The PStV-DAAA virus was poorly transmitted by aphids in vitro with HC-Pro purified from PStV or tobacco vein mottling virus (TVMV) infected plants. These experiments support the theory that specific HC-Pro/CP interactions are required for efficient aphid transmission. Based upon the sequence comparisons of 16 potyviral HC-Pro proteins several conserved motifs and striking differences have been identified. PeMoV was determined to have an Ala-Ser-Cys (ASC) HC-Pro motif instead of a highly conserved Cys-Cys-Cys (CCC) motif. We have predicted that this CCC motif could play an important role in the specific interaction between the HC-Pro and the CP DAG motif.
Starting Page	2159
Ending Page	2172
Page Count	14
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	143
Issue Number	11
e-ISSN	14328798
Language	English
Publisher	Springer-Verlag
Publisher Date	2014-04-08
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Aphid transmission of a potyvirus depends on suitability of the helper component and the N terminus of the coat protein

Coat protein sequence of Krish-infecting strain of Johnsongrass mosaic potyvirus

Reciprocal assistance for aphid transmission between non-transmissible strains of zucchini yellow mosaic potyvirus in mixed infections

Whitefly-mediated transmission of cotton leaf curl Multan betasatellite: evidence for betasatellite encapsidation in coat protein of helper begomoviruses

Expression of the potyvirus coat protein mediated by recombinant vaccinia virus and assembly of potyvirus-like particles in mammalian cells

Sequencing and characterization of the coat protein and 3′ non-coding region of a newsweet potato potyvirus

Nucleotide sequence analysis of the coat protein genes of two Korean isolates of sweet potato feathery mottle potyvirus

Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup

Sequence diversity in the coat protein coding region of twelve sugarcane mosaic potyvirus isolates from Australia, USA and South Africa

Potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency

Similar Documents

Aphid transmission of a potyvirus depends on suitability of the helper component and the N terminus of the coat protein

Coat protein sequence of Krish-infecting strain of Johnsongrass mosaic potyvirus

Reciprocal assistance for aphid transmission between non-transmissible strains of zucchini yellow mosaic potyvirus in mixed infections

Whitefly-mediated transmission of cotton leaf curl Multan betasatellite: evidence for betasatellite encapsidation in coat protein of helper begomoviruses

Expression of the potyvirus coat protein mediated by recombinant vaccinia virus and assembly of potyvirus-like particles in mammalian cells

Sequencing and characterization of the coat protein and 3′ non-coding region of a newsweet potato potyvirus

Nucleotide sequence analysis of the coat protein genes of two Korean isolates of sweet potato feathery mottle potyvirus

Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup

Sequence diversity in the coat protein coding region of twelve sugarcane mosaic potyvirus isolates from Australia, USA and South Africa

Potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency