NDLI: T cell responses to the structural polypeptides of porcine reproductive and respiratory syndrome virus

Content Provider	Springer Nature Link
Author	Bautista, E. M. Suárez, P. Molitor, T. W.
Copyright Year	1999
Abstract	The identification of antigens recognized by T cell responses has become fundamental for developing effective immunizations against viral infections. Lymphocyte proliferation and delayed-type hypersensitivity responses to porcine reproductive and respiratory syndrome virus (PRRSV) infection have been demonstrated. However, the polypeptide specificity of T cell responses to PRRSV is unknown. To identify the PRRSV polypeptides recognized by porcine lymphocytes two approaches were employed. First polypeptides of purified virions were separated by SDS-PAGE and particle suspensions obtained from nitrocellulose blots were used as antigens. Second, the polypeptides encoded by ORFs 2, 4, 5, 6, and 7 of the strain VR-2 332 were expressed as fusion proteins with a histidine tag in mammalian cells, using vaccinia virus as expression system. Significant antigen-specific proliferation responses to the matrix and envelope proteins from purified virions were obtained. This finding was supported by specific and dose-dependent proliferation responses to the recombinant polypeptides encoded by ORF2, 5 and 6 detected in virus-infected but not in control pigs. These results demonstrate that T-cell responses can be detected to individual PRRSV polypeptides. The greater response to the product of ORF6 than to the other PRRSV polypeptides indicates that the viral matrix polypeptide may have a major role in cellular immunity.
Starting Page	117
Ending Page	134
Page Count	18
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	144
Issue Number	1
e-ISSN	14328798
Language	English
Publisher	Springer-Verlag
Publisher Date	2014-04-08
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ivermectin inhibits porcine reproductive and respiratory syndrome virus in cultured porcine alveolar macrophages

Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus

DRACO inhibits porcine reproductive and respiratory syndrome virus replication in vitro

IFNγ inhibits porcine reproductive and respiratory syndrome virus replication in macrophages

Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus

Humoral immune responses and viral shedding following vaccination with modified live porcine reproductive and respiratory syndrome virus vaccines

Immune responses in mice vaccinated with virus-like particles composed of the GP5 and M proteins of porcine reproductive and respiratory syndrome virus

Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication

Porcine reproductive and respiratory syndrome virus modulates apoptosis during replication in alveolar macrophages

T cell responses to the structural polypeptides of porcine reproductive and respiratory syndrome virus

Similar Documents

Ivermectin inhibits porcine reproductive and respiratory syndrome virus in cultured porcine alveolar macrophages

Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus

DRACO inhibits porcine reproductive and respiratory syndrome virus replication in vitro

IFNγ inhibits porcine reproductive and respiratory syndrome virus replication in macrophages

Control of the PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus

Humoral immune responses and viral shedding following vaccination with modified live porcine reproductive and respiratory syndrome virus vaccines

Immune responses in mice vaccinated with virus-like particles composed of the GP5 and M proteins of porcine reproductive and respiratory syndrome virus

Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication

Porcine reproductive and respiratory syndrome virus modulates apoptosis during replication in alveolar macrophages

T cell responses to the structural polypeptides of porcine reproductive and respiratory syndrome virus