NDLI: Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors

Content Provider	Springer Nature Link
Author	Martyn, John C. Cardin, Anthony J. Wines, Bruce D. Cendron, Angela Li, Shuo Mackenzie, Jason Powell, Maree Gowans, Eric J.
Copyright Year	2009
Abstract	Recombinant baculoviruses (recBV) were constructed with dual cassettes for constitutive expression of human IgG Fc following infection of insect cells and the structural proteins of hepatitis C virus (core, E1 and E2) following transduction of mammalian cells. The IgG Fc was expressed in insect cells as a fusion protein with the signal sequence and transmembrane region of either the native baculovirus envelope protein gp64 or the human transferrin receptor as a type I or type II integral membrane protein, respectively. The IgG Fc fusion proteins formed functional homodimers on the surface of recBV-infected insect cells and were incorporated into the envelope of recBV particles during egress from the infected cell. Both pseudotyped recBV bound specifically to recombinant soluble FcγRIIα receptor and to cell lines and antigen-presenting cells expressing Fc receptors (FcRs). These novel baculoviral vectors, which target cells of the immune system that express FcRs, have potential applications for vaccination or gene therapy.
Starting Page	1129
Ending Page	1138
Page Count	10
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	154
Issue Number	7
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2009-06-26
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Infectious Diseases Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface

Binding of infectious pancreatic necrosis virus (IPNV) to membrane proteins from different fish cell lines

Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase

Construction and immunogenicity of a recombinant pseudotype baculovirus expressing the glycoprotein of rabies virus in mice

Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors

Immunoreactivity of Cytomegalovirus-Induced Fc Receptors

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Transient and stable expression of the HCV envelope glycoproteins in cell lines and primary hepatocytes transduced with a recombinant baculovirus

Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes

Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors

Similar Documents

Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface

Binding of infectious pancreatic necrosis virus (IPNV) to membrane proteins from different fish cell lines

Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase

Construction and immunogenicity of a recombinant pseudotype baculovirus expressing the glycoprotein of rabies virus in mice

Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors

Immunoreactivity of Cytomegalovirus-Induced Fc Receptors

Susceptibility of different eukaryotic cell lines to SARS-coronavirus

Transient and stable expression of the HCV envelope glycoproteins in cell lines and primary hepatocytes transduced with a recombinant baculovirus

Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes

Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors