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| Content Provider | Springer Nature Link |
|---|---|
| Author | Scheffer, Camila Mengue Varela, Ana Paula Muterle Cibulski, Samuel Paulo Schmidt, Candice Campos, Fabrício Souza Paim, Willian Pinto Santos, Raíssa Nunes Teixeira, Thais Fumaco Loiko, Márcia Regina Tochetto, Caroline Santos, Helton Fernandes Lima, Diane Alves Cerva, Cristine Mayer, Fabiana Quoos Petzhold, Sylio Alfredo Franco, Ana Cláudia George, Toby St Spilki, Fernando Rosado Roehe, Paulo Michel |
| Copyright Year | 2017 |
| Abstract | Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water buffalo (Bubalus bubalis) in 1972 in Australia. The virus was multiplied in MDBK cells, and the DNA was extracted and subjected to high-throughput sequencing. The reads were aligned and combined into a single genome sequence, with bovine alphaherpesvirus 5 (BoHV5) strain SV507/99 (accession number NC005261) as a reference. The BuHV1-b6 genome is a linear double-stranded DNA molecule, 137,452 bp long, with a GC content of 76.8%. The genome consists of two unique sequences: a long, or UL, sequence (103,818 bp) and a short, or US, sequence (9,586 bp), with the latter being flanked by inverted IR and TR elements of 12,024 bp each. The arrangement is typical of herpesvirus genomes of the D-type. The overall sequence has a 92.2% similarity at the nucleotide level to the reference BoHV5 strain. Our report provides a significant landmark in the history of herpesviruses, represented by the genome sequence of this 44-year-old virus isolate. |
| Starting Page | 1169 |
| Ending Page | 1176 |
| Page Count | 8 |
| File Format | |
| ISSN | 03048608 |
| Journal | Archives of Virology |
| Volume Number | 162 |
| Issue Number | 5 |
| e-ISSN | 14328798 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2017-01-06 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Virology Medical Microbiology Infectious Diseases |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Medicine |
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