NDLI: Subtype identification of the novel A H1N1 and other human influenza A viruses using an oligonucleotide microarray

Content Provider	Springer Nature Link
Author	Kang, Xiaoping Li, Yongqiang Sun, Honghe Wu, Weili Liu, Hong Lin, Fang Qing, Chenfeng Chang, Guohui Zhu, Qingyu Chen, Weijun Yang, Yinhui
Copyright Year	2009
Abstract	A novel strain of influenza A (H1N1) virus was isolated in Mexico and the US in March and April 2009. This novel virus spread to many countries and regions in a few months, and WHO raised the level of pandemic alert from phase 5 to phase 6 on June 11, 2009. The accurate identification of H1N1 virus and other human seasonal influenza A viruses is very important for further treatment and control of their infections. In this study, we developed an oligonucleotide microarray to subtype human H1N1, H3N2 and H5N1 influenza viruses, which could distinguish the novel H1N1 from human seasonal H1N1 influenza viruses and swine H1N1 influenza viruses. The microarray utilizes a panel of primers for multiplex PCR amplification of the hemagglutinin (HA), neuraminidase (NA) and matrix (MP) genes of human influenza A viruses. The 59-mer oligonucleotides were designed to distinguish different subtypes of human influenza A viruses. With this microarray, we accurately identified and correctly subtyped the reference virus strains. Moreover, we confirmed 4 out of 39 clinical throat swab specimens from suspected cases of novel H1N1.
Starting Page	55
Ending Page	61
Page Count	7
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	155
Issue Number	1
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2009-12-10
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Infectious Diseases Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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