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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhang, Liang Quan Zhao, Fu Rong Liu, Zhi Gang Kong, Wei Li Wang, Heng Ouyang, Yun Liang, Huan Bin Zhang, Chao Yi Qi, Hai Tao Huang, Chang Li Guo, Si Hu Zhang, Gui Hong |
| Copyright Year | 2012 |
| Abstract | Hepatitis E virus (HEV) is an enteric pathogen of humans and animals, and pigs have been considered an important reservoir of this virus. Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. In this study, we have developed a one-step RT-LAMP assay for rapid detection of swine HEV. Specific primer sets targeting the ORF3 gene were designed. The sensitivity of the RT-LAMP assay was 101 copies/μl of RNA template, which was tenfold higher than that of RT-nPCR. The specificity of this assay was demonstrated by the lack of amplification of DNA/RNA from other swine viruses. Furthermore, a total of 41 bile samples were subjected to RT-LAMP and RT-nPCR. Eighteen positive samples were detected by RT-nPCR, while 36 positive samples were detected by RT-LAMP, indicating that the sensitivity of the RT-LAMP assay was higher than that of the conventional RT-nPCR assay. The RT-LAMP assay reported here may be used for diagnosis of swine HEV, not only in laboratories but also under field conditions. |
| Starting Page | 2383 |
| Ending Page | 2388 |
| Page Count | 6 |
| File Format | |
| ISSN | 03048608 |
| Journal | Archives of Virology |
| Volume Number | 157 |
| Issue Number | 12 |
| e-ISSN | 14328798 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2012-08-02 |
| Publisher Place | Vienna |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Virology Infectious Diseases Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Medicine |
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