NDLI: Inhibition of hepatitis C virus genotype 3a by siRNAs targeting envelope genes

Content Provider	Springer Nature Link
Author	Khaliq, Saba Jahan, Shah Ijaz, Bushra Ahmad, Waqar Asad, Sultan Hassan, Sajida
Copyright Year	2010
Abstract	Hepatitis C virus (HCV) genotype 3a is considered a significant risk factor for the development of liver diseases and hepatocellular carcinoma for most of the cases in Pakistan. Because of the limited efficiency of the current therapy, RNA interference (RNAi), which results in sequence-specific degradation of HCV RNA, has potential as a powerful alternative molecular therapeutic approach. The envelope genes (E1 and E2) of HCV come in immediate contact with cells during infection and therefore might be a relevant target for new drug development. In the present study, the expression of E1 and E2 genes of HCV genotype 3a was dramatically reduced at both the mRNA and protein level using gene-specific small interfering RNAs (siRNA) when compared to mock-transfected and cells treated with control siRNAs. The potential of siRNAs to inhibit HCV-3a replication in serum-infected Huh-7 cells was also demonstrated by combined treatment of siRNAs against the E1 and E2 genes, which resulted in a significant decrease in HCV viral copy number. This clearly demonstrates that the RNAi-mediated silencing of HCV E1 and E2 is among the first of its type for the development of an effective siRNA-based therapeutic option against HCV-3a.
Starting Page	433
Ending Page	442
Page Count	10
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	156
Issue Number	3
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2010-12-16
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Inhibition of hepatitis C virus replication and expression by small interfering RNA targeting host cellular genes

Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro

Hepatitis C virus genotype 3A in a population of injecting drug users in Montenegro: Bayesian and evolutionary analysis

Hepatitis C virus genotype diversity in Shanghai, China

Inhibition of hepatitis C virus adsorption to peripheral blood mononuclear cells by dextran sulfate

An autochthonous case of hepatitis C virus genotype 5a in Brazil: phylogenetic analysis

Inhibition of Hepatitis C Virus 3a genotype entry through Glanthus Nivalis Agglutinin

Expression, immunogenicity and diagnostic value of envelope proteins from an Egyptian hepatitis C virus isolate

Effects of hepatitis C virus envelope glycoprotein unfolded protein response activation on translation and transcription

Inhibition of hepatitis C virus genotype 3a by siRNAs targeting envelope genes

Similar Documents

Inhibition of hepatitis C virus replication and expression by small interfering RNA targeting host cellular genes

Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro

Hepatitis C virus genotype 3A in a population of injecting drug users in Montenegro: Bayesian and evolutionary analysis

Hepatitis C virus genotype diversity in Shanghai, China

Inhibition of hepatitis C virus adsorption to peripheral blood mononuclear cells by dextran sulfate

An autochthonous case of hepatitis C virus genotype 5a in Brazil: phylogenetic analysis

Inhibition of Hepatitis C Virus 3a genotype entry through Glanthus Nivalis Agglutinin

Expression, immunogenicity and diagnostic value of envelope proteins from an Egyptian hepatitis C virus isolate

Effects of hepatitis C virus envelope glycoprotein unfolded protein response activation on translation and transcription

Inhibition of hepatitis C virus genotype 3a by siRNAs targeting envelope genes