NDLI: Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand

Content Provider	Springer Nature Link
Author	Wilburn, Lauren Yodmeeklin, Arpaporn Kochjan, Pakawat Saikruang, Wilaiporn Kumthip, Kattareeya Khamrin, Pattara Maneekarn, Niwat
Copyright Year	2016
Abstract	Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonomically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. To determine the prevalence and genetic diversity of porcine PBVs in Northern Thailand, 380 fecal samples collected from diarrheic and non-diarrheic piglets, raised in 22 pig farms, were tested for the presence of PBV. Reverse-transcription PCR (RT-PCR) was performed using primer sets specific to the RNA-dependent RNA polymerase (RdRp) gene. PBV was detected in 86 of 265 (32.5%) diarrheic piglets and in 26 of 115 (22.6%) non-diarrheic piglets. All the PBV strains detected in this study belonged to genogroup I and a high proportion of PBV-positive piglets were co-infected with group A rotavirus (RVA) and bocavirus (BoV). Phylogenetic analysis of representative genogroup I strains revealed remarkably high similarity between strains; these formed a monophyletic cluster with 97-100% sequence identity in the RdRp gene. The strains were also closely related to genogroup I PBV Chinese porcine strain. The findings indicate that PBV infection is common in piglets with and without diarrhea in Northern Thailand.
Starting Page	1061
Ending Page	1066
Page Count	6
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	162
Issue Number	4
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2016-12-28
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Picobirnavirus Diarrhea Thailand Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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