NDLI: Combined amino acid mutations occurring in the envelope closely correlate with pathogenicity of EIAV

Content Provider	Springer Nature Link
Author	Liang, H. He, X. Shen, R. X. Shen, T. Tong, X. Ma, Y. Xiang, W. H. Zhang, X. Y. Shao, Y. M.
Copyright Year	2006
Abstract	The Chinese equine infectious anemia virus (EIAV) donkey-leukocyte attenuated vaccine (DLV) provides a unique natural model system to study the attenuation mechanism and immunological control of lentivirus replication. Critical consensus mutations were identified between virulent Chinese EIAV strains and vaccine strains. Based on a full-length infectious clone of EIAV vaccine strain pLGFD3, two molecular clones, mFD5-4-7 and mFD7-2-11, were successfully constructed, in which 4 and 6 critical consensus mutations in the env gene of the vaccine strain were point-mutated to the wild-type sequence, respectively by an overlap PCR mutagenesis strategy. The infectivity, virulence, and pathogenesis of the constructed clones were investigated in vitro using a reverse transcriptase assay, an indirect immunofluorescence assay, observation of cytopathogenic effect, and virion observation as well as in vivo by inoculation of animals with the resulting infectious clones. The pathogenic symptoms in horses inoculated with mFD7-2-11 were more severe than those inoculated with mFD5-4-7, whereas no pathogenic symptoms were detected in animals inoculated with their parental clone pLGFD3 strain. The results indicate that the consensus mutation residues of the env region involved in this study play significant roles in the virulence and pathogenicity of EIAV. This will contribute to the elucidation of the attenuating and protective mechanisms of the Chinese EIAV vaccine.
Starting Page	1387
Ending Page	1403
Page Count	17
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	151
Issue Number	7
e-ISSN	14328798
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-02-26
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Infectious Diseases Virology
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Amino acid substitutions occurring during adaptation of an emergent H5N6 avian influenza virus to mammals

Nucleotide and amino acid mutations in human immunodeficiency virus corresponding to CD4+ decline

Three amino acid changes in PB1-F2 of highly pathogenic H5N1 avian influenza virus affect pathogenicity in mallard ducks

Hepatitis C virus amino acid sequence diversity correlates with the outcome of combined interferon/ribavirin therapy in Chinese patients with chronic hepatitis C

A single substitution in amino acid 184 of the NP protein alters the replication and pathogenicity of H5N1 avian influenza viruses in chickens

Luteovirus: insights into pathogenicity

Infectious bronchitis viruses with naturally occurring genomic rearrangement and gene deletion

Unique evolution characteristics of the envelope protein of EIAV$_{LN40}$, a virulent strain of equine infectious anemia virus

A pilot study on an attenuated Chinese EIAV vaccine inducing broadly neutralizing antibodies

Combined amino acid mutations occurring in the envelope closely correlate with pathogenicity of EIAV

Similar Documents

Amino acid substitutions occurring during adaptation of an emergent H5N6 avian influenza virus to mammals

Nucleotide and amino acid mutations in human immunodeficiency virus corresponding to CD4+ decline

Three amino acid changes in PB1-F2 of highly pathogenic H5N1 avian influenza virus affect pathogenicity in mallard ducks

Hepatitis C virus amino acid sequence diversity correlates with the outcome of combined interferon/ribavirin therapy in Chinese patients with chronic hepatitis C

A single substitution in amino acid 184 of the NP protein alters the replication and pathogenicity of H5N1 avian influenza viruses in chickens

Luteovirus: insights into pathogenicity

Infectious bronchitis viruses with naturally occurring genomic rearrangement and gene deletion

Unique evolution characteristics of the envelope protein of EIAV$_{LN40}$, a virulent strain of equine infectious anemia virus

A pilot study on an attenuated Chinese EIAV vaccine inducing broadly neutralizing antibodies

Combined amino acid mutations occurring in the envelope closely correlate with pathogenicity of EIAV