NDLI: Genetic variation and cultivar identification in Cymbidium ensifolium

Content Provider	Springer Nature Link
Author	Wang, Hui Zhong Lu, Jiang Jie Hu, Xu Liu, Jun Jun
Copyright Year	2011
Abstract	As a popular flowering species with many cultivars, Cymbidium ensifolium (L.) is commercially important in horticulture. However, so far little has been known about genetic diversity and conservation genetics of this species. Understanding of the genetic variation and relationships in cultivars of C. ensifolium is a prerequisite for development of future germplasm conservation and cultivar improvement. Here we report assessment of genetic variations in C. ensifolium cultivars using the DNA fingerprinting technique of inter-simple sequence repeats (ISSR). A total of 239 ISSR loci were identified and used for evaluation of genetic variation with a selection of 19 ISSR primers. Among these ISSR loci, 99.16% were polymorphic with wide genetic variation as shown by Nei’s gene diversity (H = 0.2431) among 85 tested cultivars. ISSR fingerprinting profiles showed that each cultivar had its characteristic DNA pattern, indicating unequivocal cultivar identification at molecular level. Eighteen cultivar-specific ISSR markers were identified in seven cultivars. The cultivar Sijiwenhan was confirmed as hybrid by four ISSR primers. Several cultivars with same name but different geographical origins were distinguished based on their ISSR profiles. A dendrogram generated with ISSR markers could group 73 of 85 cultivars into four major clusters. Further analysis of ISSR variation revealed that about 69% of total genetic variation in this species is due to genetic divergence inside geographical groups. Our results suggest that both germplasm collection and in situ conservation are important for future planning of C. ensifolium species conservation.
Starting Page	101
Ending Page	110
Page Count	10
File Format	PDF
ISSN	03782697
Journal	Plant Systematics and Evolution
Volume Number	293
Issue Number	1-4
e-ISSN	16156110
Language	English
Publisher	Springer Vienna
Publisher Date	2011-03-10
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cultivar Cymbidium ensifolium Genetic variation Inter-simple sequence repeat Plant Ecology Plant Sciences Plant Systematics/Taxonomy/Biogeography Plant Anatomy/Development
Content Type	Text
Resource Type	Article
Subject	Plant Science Ecology, Evolution, Behavior and Systematics

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