NDLI: Toehold-aided DNA recycling amplification using hemin and G-quadruplex reporter DNA on magnetic beads as tags for chemiluminescent determination of riboflavin

Content Provider	Springer Nature Link
Author	Meng, Yan Hun, Xu Zhang, Yue Luo, Xiliang
Copyright Year	2016
Abstract	A toehold-aided DNA recycling amplification technology was developed based on the combination of toehold-aided DNA recycling and the hemin/G-quadruplex label. The dsDNA formed between aptamer and DNA1 was first immobilized on magnetic beads. On addition of target analyte (exemplified here for riboflavin), the aptamer-riboflavin complex is formed and DNA1 is released by the beads. After magnetic separation, the supernatant containing the released DNA1 is added to a solution containing the hairpin capture DNA on magnetic beads. DNA1 will hybridize with the hairpin capture DNA via toehold binding and branch migration. This process will open the hairpin structure, and an external toehold is formed in the newly formed dsDNA. On addition of reporter DNA containing the G-quadruplex, it will interact with the formed dsDNA via toehold binding and branch migration, resulting in the releasing of DNA1 and capturing of reporter DNA on the magnetic beads. The released DNA1 will bind to another hairpin capture DNA which can start another round of DNA1 recycling. Chemiluminescence (CL) is generated by the G-quadruplex-hemin-luminol CL reaction system. Under optimal conditions, the calibration plot is linear in the 0.1 to 700 nM riboflavin concentration range, with a 30 pM detection limit (at a signal-to-noise ratio of 3). The method was successfully applied to the quantitation of riboflavin in spiked urine samples. Graphical abstract Toehold-aided DNA recycling coupled with hemin G-quadruplex for target detection.
Starting Page	2965
Ending Page	2971
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	183
Issue Number	11
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-09-08
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Toehold amplification Aptamer Chemiluminescence Luminol Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Chemiluminescent determination of the activity of caspase-3 using a specific peptide substrate and magnetic beads

Colorimetric and visual determination of melamine by exploiting the conformational change of hemin G-quadruplex-DNAzyme

A voltammetric aptamer-based thrombin biosensor exploiting signal amplification via synergetic catalysis by DNAzyme and enzyme decorated AuPd nanoparticles on a poly(o-phenylenediamine) support

Chemiluminescence assay for the glycoprotein tenascin-C based on aptamer-modified carboxylated magnetic carbon nanoparticles

Colorimetric determination of staphylococcal enterotoxin B via DNAzyme-guided growth of gold nanoparticles

Silicon-hybrid carbon dots strongly enhance the chemiluminescence of luminol

Ultra-sensitive chemiluminescent detection of Staphylococcus aureus based on competitive binding of Staphylococcus protein A-modified magnetic beads to immunoglobulin G

A fluorescent sandwich assay for thrombin using aptamer modified magnetic beads and quantum dots

G-quadruplex − based homogenous fluorescence platform for ultrasensitive DNA detection through isothermal cycling and cascade signal amplification

Toehold-aided DNA recycling amplification using hemin and G-quadruplex reporter DNA on magnetic beads as tags for chemiluminescent determination of riboflavin

Similar Documents

Chemiluminescent determination of the activity of caspase-3 using a specific peptide substrate and magnetic beads

Colorimetric and visual determination of melamine by exploiting the conformational change of hemin G-quadruplex-DNAzyme

A voltammetric aptamer-based thrombin biosensor exploiting signal amplification via synergetic catalysis by DNAzyme and enzyme decorated AuPd nanoparticles on a poly(o-phenylenediamine) support

Chemiluminescence assay for the glycoprotein tenascin-C based on aptamer-modified carboxylated magnetic carbon nanoparticles

Colorimetric determination of staphylococcal enterotoxin B via DNAzyme-guided growth of gold nanoparticles

Silicon-hybrid carbon dots strongly enhance the chemiluminescence of luminol

Ultra-sensitive chemiluminescent detection of Staphylococcus aureus based on competitive binding of Staphylococcus protein A-modified magnetic beads to immunoglobulin G

A fluorescent sandwich assay for thrombin using aptamer modified magnetic beads and quantum dots

G-quadruplex − based homogenous fluorescence platform for ultrasensitive DNA detection through isothermal cycling and cascade signal amplification

Toehold-aided DNA recycling amplification using hemin and G-quadruplex reporter DNA on magnetic beads as tags for chemiluminescent determination of riboflavin