NDLI: Manipulation of the Electroosmotic Flow in Glass und PMMA Microchips with Respect to Specific Enzymatic Glucose Determinations

Content Provider	Springer Nature Link
Author	Mersal, Gaber A. M. Bilitewski, Ursula
Copyright Year	2005
Abstract	The determination of glucose in microfluidic chips made of glass or PMMA was used as a model for the combination of an enzymatic reaction with the separation of compounds. It was based on the enzymatic oxidation of glucose and the amperometric detection of hydrogen peroxide. Real samples frequently contain compounds, such as ascorbic acid, which may interfere with quantitative glucose determinations. Thus, electrophoretic separation of specific from unspecific signals was envisaged by applying electric fields which are also used to control the flow of liquid via electroosmotic effects. Surface charge densities of the capillaries influence the electroosmotic flow (EOF). They are dependent on the chip material and on the adsorption of components from the background electrolyte. Reversal of the EOF after addition of cetyltrimethylammonium bromide (CTAB) and an increase in EOF after addition of sodium dodecylsulfate (SDS) were observed at lower surfactant concentrations with the PMMA chips rather than with the glass chips. For both chip materials these concentrations were below the critical micelle concentration. Effective separation of H2O2 and ascorbic acid was achieved with low CTAB concentrations, which lead to a reduction, but not to a reversal of the EOF. Reversal of the EOF by higher CTAB concentrations or the increase in cathodic EOF by SDS accelerated ascorbic acid transportation and reduced the differences in migration times. Thus, for the specific determination of glucose, glucose oxidase was added together with low CTAB concentrations to the background electrolyte. This avoided interference from ascorbic acid, and data obtained from the analysis of fruit juices showed a good correlation to data obtained from a reference method.
Starting Page	29
Ending Page	38
Page Count	10
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	151
Issue Number	1-2
e-ISSN	14365073
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-08-01
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Inorganic Chemistry Physical Chemistry Monitoring/Environmental Analysis/Environmental Ecotoxicology Analytical Chemistry Characterization and Evaluation Materials
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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Manipulation of the Electroosmotic Flow in Glass und PMMA Microchips with Respect to Specific Enzymatic Glucose Determinations

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Manipulation of the Electroosmotic Flow in Glass und PMMA Microchips with Respect to Specific Enzymatic Glucose Determinations